MCR
{

RETE|ID 1 FBmc0001377	CCL 1 constr	SGTP 1 -	SYM 1 :pP{Rh1(-162/+67)-CAT-Rh1(-219/-501)}	DBA 1 -	REF 1 1	DT 1 21 May 1998	RESZ 360
ID|FBmc0001377
SYM|:pP{Rh1(-162/+67)-CAT-Rh1(-219/-501)}
DT|21 May 1998
|21 Mar 1997
CCL|constr
ID2|FBmc0000537

REFDSR
{
RDID|FBrf0046388
|Mismer and Rubin
|1987
TPU|characterization | ninaE
|reporter construct | ninaE
PRG|in vitro progenitor: <fbsym>pP{Rh1(-501/-219,&Dgr;,-162/+67)-CAT}</fbsym> precursor
}

REF
{
REFM|FBrf0046388
|Mismer and Rubin
|1987
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000579	CCL 1 constr	SGTP 1 composite	SYM 1 CosC4	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 515
ID|FBmc0000579
SYM|CosC4
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0046973
|Mariol et al.
|1987
SYN|CosC4
ASAL|fu<up>+CosC4</up>
TCL|cosmid
TPU|characterization | fu
|transient assay | embryo | fu
CC|construct
|Cosmid containing ~39kb genomic sequences from the <fbsym>fused</fbsym> region.  Used in
| transient rescue assays involving injection of DNA into early embryos
| followed by analysis of cuticular phenotypes in late embryos.
}

REF
{
REFM|FBrf0046973
|Mariol et al.
|1987
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000135	CCL 1 constr	SGTP 1 composite	SYM 1 cosP	DBA 1 -	REF 1 3	DT 1 8 Apr 1997	RESZ 1131
ID|FBmc0000135
SYM|cosP
SYN|cosP{6.1C}
TRL|~7512
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 + <fbsym>FBms0000244==6.1_P5'(P5',X1)-Pv2</fbsym>
|2 + <fbsym>FBms0000245==001071</fbsym>
|3 + <fbsym>FBms0000599==Car1_P3'(H3,P3')</fbsym>
|4 + <fbsym>FBms0003682==cosneo-P</fbsym>
MMP|FBmc0000135
RSQ|FBmc0000135

REFDSR
{
RDID|FBrf0042056
|Haenlin et al.
|1985
DEC|in vitro descendant <fbsym>FBmc0000242==cosP{K10-9}</fbsym>
|in vitro descendant <fbsym>FBmc0000243==cosP{K10-15}</fbsym>
}

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
CS|BamHI
TCL|cosmid
TFT|lambda cos sites (3)
TPU|cloning vector | cosmid
|cloning vector | transposon | P-element
PRG|in vitro progenitor <fbsym>FBmc0000103==pP{6.1}</fbsym>
|in vitro progenitor <fbsym>FBmc0000112==pP{Car1}</fbsym>
|in vitro progenitor: cos4 (FBrf0039298)
DEC|in vitro descendant <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|transposon descendant <fbsym>FBtp0001051==P{6.1C}</fbsym>
}

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
CS|BamHI
}

REF
{
REFM|FBrf0042056
|Haenlin et al.
|1985
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000359	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{5UZ-neo}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 1243
ID|FBmc0000359
SYM|cosP{5UZ-neo}
SYN|cPnbetabbxd6.2
|cPnbetabxd5
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000357==cosP{22UZ}</fbsym>
|<fbsym>FBmc0000356==cosP{35UZ}</fbsym>
|<fbsym>FBmc0000359==cosP{5UZ-neo}</fbsym>
|<fbsym>FBmc0000358==cosP{5UZ}</fbsym>
ASTR|<fbsym>FBtr0003751==Ecol\lacZ<up>Ubx.5UZ</up>RA</fbsym>
ASGN|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
SYN|cPnbetabxd5
ASAL|<fbsym>FBal0042141==Ecol\lacZ<up>Ubx.5UZ</up></fbsym>
|neoR<up>Hsp70Ba.PS</up>
TCL|cosmid
TFT|chimeric gene | Hsp70Ba::Tn\neoR
|reporter gene | <fbsym>FBal0042141==Ecol\lacZ[Ubx.5UZ]</fbsym>
|selectable marker | <fbsym>neoR<up>Hsp70Ba.PS</up></fbsym>
|chimeric gene | Ubx::Ecol\lacZ::Simian virus 40
TPU|reporter construct | Ubx
|characterization | Ubx | regulatory
CC|construct
|One of a series of reporter gene constructs used to characterize the <fbsym>FBgn0003944==Ubx</fbsym>
| UCR (upstream control region).
PRG|in vitro progenitor <fbsym>FBmc0000352==cosP{neo&bgr;-gal}</fbsym>
|in vitro progenitor: <fbsym>FBgn0003944==Ubx</fbsym> region genomic DNA (FBrf0053791)
DEC|transposon descendant <fbsym>FBtp0000330==P{5UZ-neo}</fbsym>
}

REF
{
REFM|FBrf0053791
|Irvine et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000358	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{5UZ}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 1243
ID|FBmc0000358
SYM|cosP{5UZ}
SYN|cPwbetabxd5
|cPwbetabxd6.2
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000357==cosP{22UZ}</fbsym>
|<fbsym>FBmc0000356==cosP{35UZ}</fbsym>
|<fbsym>FBmc0000359==cosP{5UZ-neo}</fbsym>
|<fbsym>FBmc0000358==cosP{5UZ}</fbsym>
ASTR|<fbsym>FBtr0003751==Ecol\lacZ<up>Ubx.5UZ</up>RA</fbsym>
ASGN|<fbsym>FBgn0003996==w</fbsym>
|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
SYN|cPwbetabxd5
ASAL|<fbsym>FBal0042141==Ecol\lacZ<up>Ubx.5UZ</up></fbsym>
|<fbsym>FBal0028610==w<up>+mC</up></fbsym>
TCL|cosmid
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|reporter gene | <fbsym>FBal0042141==Ecol\lacZ[Ubx.5UZ]</fbsym>
|chimeric gene | Ubx::Ecol\lacZ::Simian virus 40
TPU|reporter construct | Ubx
|characterization | Ubx | regulatory
CC|construct
|One of a series of reporter gene constructs used to characterize the <fbsym>FBgn0003944==Ubx</fbsym>
| UCR (upstream control region).
PRG|in vitro progenitor <fbsym>FBmc0000355==cosP{w&bgr;-gal}</fbsym>
|in vitro progenitor: <fbsym>FBgn0003944==Ubx</fbsym> region genomic DNA (FBrf0053791)
DEC|transposon descendant <fbsym>FBtp0000329==P{5UZ}</fbsym>
}

REF
{
REFM|FBrf0053791
|Irvine et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000357	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{22UZ}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 1206
ID|FBmc0000357
SYM|cosP{22UZ}
SYN|cPwbetabxd22
|cPwbetabxd23
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000357==cosP{22UZ}</fbsym>
|<fbsym>FBmc0000356==cosP{35UZ}</fbsym>
|<fbsym>FBmc0000358==cosP{5UZ}</fbsym>
ASTR|<fbsym>FBtr0003750==Ecol\lacZ<up>Ubx.22UZ</up>RA</fbsym>
ASGN|<fbsym>FBgn0003996==w</fbsym>
|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
SYN|cPwbetabxd22
ASAL|<fbsym>FBal0042136==Ecol\lacZ<up>Ubx.22UZ</up></fbsym>
|<fbsym>FBal0028610==w<up>+mC</up></fbsym>
TCL|cosmid
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|reporter gene | <fbsym>FBal0042136==Ecol\lacZ[Ubx.22UZ]</fbsym>
|chimeric gene | Ubx::Ecol\lacZ::Simian virus 40
TPU|reporter construct | Ubx
|characterization | Ubx | regulatory
CC|construct
|One of a series of reporter gene constructs used to characterize the <fbsym>FBgn0003944==Ubx</fbsym>
| UCR (upstream control region).
PRG|in vitro progenitor <fbsym>FBmc0000355==cosP{w&bgr;-gal}</fbsym>
|in vitro progenitor: <fbsym>FBgn0003944==Ubx</fbsym> region genomic DNA (FBrf0053791)
DEC|transposon descendant <fbsym>FBtp0000328==P{22UZ}</fbsym>
}

REF
{
REFM|FBrf0053791
|Irvine et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000356	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{35UZ}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 1206
ID|FBmc0000356
SYM|cosP{35UZ}
SYN|cPwbetabxd35
|cPwbetabxd36
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000357==cosP{22UZ}</fbsym>
|<fbsym>FBmc0000356==cosP{35UZ}</fbsym>
|<fbsym>FBmc0000358==cosP{5UZ}</fbsym>
ASTR|<fbsym>FBtr0003656==Ecol\lacZ<up>Ubx.35UZ</up>RA</fbsym>
ASGN|<fbsym>FBgn0003996==w</fbsym>
|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
SYN|cPwbetabxd35
ASAL|<fbsym>FBal0042137==Ecol\lacZ<up>Ubx.35UZ</up></fbsym>
|<fbsym>FBal0028610==w<up>+mC</up></fbsym>
TCL|cosmid
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|reporter gene | <fbsym>FBal0042137==Ecol\lacZ[Ubx.35UZ]</fbsym>
|chimeric gene | Ubx::Ecol\lacZ::Simian virus 40
TPU|reporter construct | Ubx
|characterization | Ubx | regulatory
CC|construct
|One of a series of reporter gene constructs used to characterize the <fbsym>FBgn0003944==Ubx</fbsym>
| UCR (upstream control region).
PRG|in vitro progenitor <fbsym>FBmc0000355==cosP{w&bgr;-gal}</fbsym>
|in vitro progenitor: <fbsym>FBgn0003944==Ubx</fbsym> region genomic DNA (FBrf0053791)
DEC|transposon descendant <fbsym>FBtp0000327==P{35UZ}</fbsym>
}

REF
{
REFM|FBrf0053791
|Irvine et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000363	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{bw<up>+</up>1111}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 919
ID|FBmc0000363
SYM|cosP{bw<up>+</up>1111}
TRL|39000
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0000241==bw</fbsym>

REFDSR
{
RDID|FBrf0054058
|Dreesen et al.
|1991
ASAL|<fbsym>FBal0034317==bw<up>+t30</up></fbsym>
|neoR<up>Hsp70Ba.PS</up>
TCL|cosmid
TFT|complete rescue | <fbsym>FBal0034317==bw[+t30]</fbsym>
|selectable marker | <fbsym>neoR<up>Hsp70Ba.PS</up></fbsym>
TPU|characterization | <fbsym>FBgn0000241==bw</fbsym>
|recombinant cloning construct | <fbsym>FBgn0000241==bw</fbsym>
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|in vitro progenitor: <fbsym>FBgn0000241==bw</fbsym> genomic region DNA (FBrf0048667)
DEC|in vitro descendant <fbsym>FBmc0000361==pP{bw<up>+</up>L}</fbsym>
|in vitro descendant <fbsym>FBmc0000362==pP{bw<up>+</up>R}</fbsym>
|transposon descendant <fbsym>FBtp0001426==P{bw<up>+</up>1111}</fbsym>
}

REF
{
REFM|FBrf0054058
|Dreesen et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000912	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{cosh}	DBA 1 -	REF 1 1	DT 1 29 Dec 1997	RESZ 753
ID|FBmc0000912
SYM|cosP{cosh}
DT|29 Dec 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0001168==h</fbsym>
|<fbsym>FBgn0014449==Tn\neoR</fbsym>

REFDSR
{
RDID|FBrf0049623
|Rushlow et al.
|1989
ASAL|<fbsym>FBal0031446==h<up>cosh</up></fbsym>
|<fbsym>FBal0042319==Tn\neoR<up>Hsp70Bb.PS</up></fbsym>
TCL|cosmid
TFT|partial rescue | <fbsym>FBal0031446==h[cosh]</fbsym>
|selectable marker | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
TPU|characterization | <fbsym>FBgn0001168==h</fbsym>
PRG|in vitro progenitor <fbsym>FBmc0000913==cosP{neoR.A}</fbsym>
|in vitro progenitor: <fbsym>FBgn0001168==h</fbsym> region genomic sequences
DEC|transposon descendant <fbsym>FBtp0000860==P{cosh}</fbsym>
}

REF
{
REFM|FBrf0049623
|Rushlow et al.
|1989
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000234	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{cosPer}	DBA 1 -	REF 1 7	DT 1 8 Apr 1997	RESZ 1910
ID|FBmc0000234
SYM|cosP{cosPer}
SYN|cosPer
TRL|11542
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 + <fbsym>FBms0000065==Car1_P5'(P5',R1)</fbsym>
|2 + <fbsym>FBms0000726==001105</fbsym>
|3 - <fbsym>FBms0000515==w<up>+mC</up>4.1(Sp)</fbsym>
|4 + <fbsym>FBms0000517==001068</fbsym>
|5 + <fbsym>FBms0000086==M13mp10mcs</fbsym>
|6 + <fbsym>FBms0000599==Car1_P3'(H3,P3')</fbsym>
|7 + <fbsym>FBms0003682==cosneo-P</fbsym>
ASGN|<fbsym>FBgn0003996==w</fbsym>
MMP|FBmc0000234
RSQ|FBmc0000234

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
TFT|lambda cos sites (3)
}

REFDSR
{
RDID|FBrf0048982
|Pirrotta
|1988
ASAL|<fbsym>FBal0028610==w<up>+mC</up></fbsym>
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|partial rescue | mini-gene::<fbsym>FBal0028610==w[+mC]</fbsym>
}

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
DEC|in vitro descendant <fbsym>FBmc0000355==cosP{w&bgr;-gal}</fbsym>
}

REFDSR
{
RDID|FBrf0068449
|Chou et al.
|1993
DEC|in vitro descendant <fbsym>FBmc0000387==cosP{ovoD1-S23-1}</fbsym>
}

REFDSR
{
RDID|FBrf0074787
|White et al.
|1994
DEC|in vitro descendant <fbsym>FBmc0001361==cosP{NT1B1}</fbsym>
|in vitro descendant <fbsym>FBmc0001362==cosP{HT3C}</fbsym>
}

REFDSR
{
RDID|FBrf0075322
|Pirrotta
|1994
SYN|cosPer
TCL|cosmid
TPU|cloning vector | cosmid
|cloning vector | transposon | P-element
CC|construct
|Most of the cloning sites in <fbsym>FBmc0000136==cosP{neoR}</fbsym> should also be unique in this vector.
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|in vitro progenitor <fbsym>FBmc0000168==pP{CaSpeR}</fbsym>
DEC|transposon descendant <fbsym>FBtp0000214==P{cosPer}</fbsym>
}

REF
{
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0048982
|Pirrotta
|1988
REFM|FBrf0053791
|Irvine et al.
|1991
REFM|FBrf0068449
|Chou et al.
|1993
REFM|FBrf0074787
|White et al.
|1994
REFM|FBrf0075322
|Pirrotta
|1994
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000538	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{HSH3}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 1300
ID|FBmc0000538
SYM|cosP{HSH3}
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000538==cosP{HSH3}</fbsym>
|<fbsym>FBmc0000539==cosP{HSH4}</fbsym>
|<fbsym>FBmc0000536==pP{HSH1}</fbsym>
|<fbsym>FBmc0002679==pP{HSH2}</fbsym>
ASGN|<fbsym>FBgn0001168==h</fbsym>

REFDSR
{
RDID|FBrf0045759
|Ish-Horowicz and Pinchin
|1987
ASAL|<fbsym>FBal0031448==h<up>hs.P1</up></fbsym>
|neoR<up>Hsp70Bb.PS</up>
TCL|cosmid
TFT|general promoter | <fbsym>FBal0031448==h[hs.P1]</fbsym>
|chimeric gene | Hsp70*::Ig::<fbsym>FBgn0001168==h</fbsym>
|inducible promoter | <fbsym>FBal0031448==h[hs.P1]</fbsym>
|selectable marker | <fbsym>neoR<up>Hsp70Bb.PS</up></fbsym>
TPU|characterization | <fbsym>FBgn0001168==h</fbsym>
CC|construct
|One of a series of heat-shock-hairy constructs cloned into <fbsym>neoR</fbsym> vectors.
|report
|Ig = unspecified immunoglobulin sequences.
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|in vitro progenitor: Ig sequences (unspecified)
|in vitro progenitor: <fbsym>FBgn0001168==h</fbsym> cDNA sequences
|in vitro progenitor: <fbsym>FBgn0001168==h</fbsym> genomic sequences
|in vitro progenitor: <fbsym>Hsp70*</fbsym> genomic sequences
DEC|transposon descendant <fbsym>FBtp0000489==P{HSH3}</fbsym>
}

REF
{
REFM|FBrf0045759
|Ish-Horowicz and Pinchin
|1987
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000539	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{HSH4}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 1287
ID|FBmc0000539
SYM|cosP{HSH4}
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000538==cosP{HSH3}</fbsym>
|<fbsym>FBmc0000539==cosP{HSH4}</fbsym>
|<fbsym>FBmc0000536==pP{HSH1}</fbsym>
|<fbsym>FBmc0002679==pP{HSH2}</fbsym>
ASGN|<fbsym>FBgn0001168==h</fbsym>

REFDSR
{
RDID|FBrf0045759
|Ish-Horowicz and Pinchin
|1987
ASAL|<fbsym>FBal0045831==h<up>hs.P2</up></fbsym>
|neoR<up>Hsp70Bb.PS</up>
TCL|cosmid
TFT|general promoter | <fbsym>FBal0045831==h[hs.P2]</fbsym>
|inducible promoter | <fbsym>FBal0045831==h[hs.P2]</fbsym>
|chimeric gene | Hsp70*::Ig::h::Hsp70Ab
|selectable marker | <fbsym>neoR<up>Hsp70Bb.PS</up></fbsym>
TPU|characterization | <fbsym>FBgn0001168==h</fbsym>
CC|construct
|One of a series of heat-shock-hairy constructs cloned into <fbsym>neoR</fbsym> vectors.
|report
|Ig = unspecified immunoglobulin sequences.
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|in vitro progenitor: Ig sequences (unspecified)
|in vitro progenitor: <fbsym>FBgn0001168==h</fbsym> cDNA sequences
|in vitro progenitor: <fbsym>Hsp70*</fbsym> genomic sequences
|in vitro progenitor: <fbsym>FBgn0013276==Hsp70Ab</fbsym> genomic sequences
DEC|transposon descendant <fbsym>FBtp0000490==P{HSH4}</fbsym>
}

REF
{
REFM|FBrf0045759
|Ish-Horowicz and Pinchin
|1987
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001362	CCL 1 constr	SGTP 1 -	SYM 1 cosP{HT3C}	DBA 1 -	REF 1 1	DT 1 8 Jun 1998	RESZ 358
ID|FBmc0001362
SYM|cosP{HT3C}
DT|8 Jun 1998
|9 Apr 1998
CCL|constr

REFDSR
{
RDID|FBrf0074787
|White et al.
|1994
TPU|control construct
PRG|in vitro progenitor <fbsym>FBmc0000234==cosP{cosPer}</fbsym>
|in vitro progenitor: 75C1,2 genomic sequences
DEC|transposon descendant <fbsym>FBtp0001387==P{HT3C}</fbsym>
}

REF
{
REFM|FBrf0074787
|White et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000242	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{K10-9}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 1086
ID|FBmc0000242
SYM|cosP{K10-9}
SYN|cos9
TRL|~46500
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0000377==crn</fbsym>
|<fbsym>FBgn0000810==fs(1)K10</fbsym>
|<fbsym>FBgn0001330==kz</fbsym>
|<fbsym>FBgn0003048==pcx</fbsym>

REFDSR
{
RDID|FBrf0042056
|Haenlin et al.
|1985
SYN|cos9
ASAL|<fbsym>FBal0034373==crn<up>+tcos9</up></fbsym>
|<fbsym>FBal0034638==fs(1)K10<up>+tcos9</up></fbsym>
|<fbsym>FBal0034821==kz<up>+tcos9</up></fbsym>
|<fbsym>FBal0035352==pcx<up>+tcos9</up></fbsym>
TCL|cosmid
TFT|complete rescue | <fbsym>FBal0034821==kz[+tcos9]</fbsym>
|complete rescue | <fbsym>FBal0034373==crn[+tcos9]</fbsym>
|complete rescue | <fbsym>FBal0035352==pcx[+tcos9]</fbsym>
|complete rescue | <fbsym>FBal0034638==fs(1)K10[+tcos9]</fbsym>
TPU|characterization | <fbsym>FBgn0000810==fs(1)K10</fbsym>
PRG|in vitro progenitor <fbsym>FBmc0000135==cosP</fbsym>
|in vitro progenitor: genomic DNA from region of <fbsym>FBgn0000810==fs(1)K10</fbsym> (FBrf0042056)
DEC|transposon descendant <fbsym>FBtp0000222==P{K10-9}</fbsym>
}

REF
{
REFM|FBrf0042056
|Haenlin et al.
|1985
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000243	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{K10-15}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 557
ID|FBmc0000243
SYM|cosP{K10-15}
SYN|cos15
TRL|~50000
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0042056
|Haenlin et al.
|1985
SYN|cos15
TCL|cosmid
TPU|characterization | fs(1)K10
CC|construct
|Attempted selection of transformants by ability to rescue sterility of
| fs(1)K10; no transformants recovered.
PRG|in vitro progenitor <fbsym>FBmc0000135==cosP</fbsym>
|in vitro progenitor: genomic DNA from region of (or adjacent to) <fbsym>FBgn0000810==fs(1)K10</fbsym> (FBrf0042056)
}

REF
{
REFM|FBrf0042056
|Haenlin et al.
|1985
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000353	CCL 1 -	SGTP 1 composite	SYM 1 cosP{neo&bgr;-b}	DBA 1 -	REF 1 5	DT 1 7 May 1999	RESZ 2769
ID|FBmc0000353
SYM|cosP{neo&bgr;-b}
SYN|cPnbetab
TRL|~13070
DT|7 May 1999
|21 Mar 1997
SGTP|composite
SEG|1 + <fbsym>FBms0000249==cosPneo_P5'(P5',R1)</fbsym>
|2 + <fbsym>FBms0003596==001236</fbsym>
|3 - <fbsym>FBms0000693==lacZ::SV40_4.2(-P)</fbsym>
|4 - <fbsym>FBms0003597==001238</fbsym>
|5 + <fbsym>FBms0000599==Car1_P3'(H3,P3')</fbsym>
|6 + <fbsym>FBms0003684==cosneo&bgr;-P</fbsym>
ASCO|<fbsym>FBmc0000353==cosP{neo&bgr;-b}</fbsym>
|<fbsym>FBmc0000354==cosP{neo&bgr;-c}</fbsym>
|<fbsym>FBmc0000352==cosP{neo&bgr;-gal}</fbsym>
ASGN|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>
|<fbsym>FBgn0014449==Tn\neoR</fbsym>
MMP|FBmc0000353
RSQ|FBmc0000353

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
ASAL|<fbsym>FBal0042319==Tn\neoR<up>Hsp70Bb.PS</up></fbsym>
TFT|lambda cos sites (3)
|allows selection with G418
|bacterial selection | ampR
|chimeric gene | Hsp70Bb::neoR
|chimeric gene | Hsp70Bb::<fbsym>FBgn0014449==Tn\neoR</fbsym>
|general promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|selectable marker | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|inducible promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|reporter cassette (-ATG) | lacZ<up>C4</up>
|selectable marker | neoR<up>Hsp70Bb.PS</up>
}

REFDSR
{
RDID|FBrf0048083
|Thummel et al.
|1988
ASAL|<fbsym>FBal0040953==Ecol\lacZ<up>C4</up></fbsym>
}

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
CS|In front of <fbsym>lacZ</fbsym> reporter cassette: SalI <up>KpnI</up> XbaI BamHI SmaI
SYN|cPnbetab
ASAL|neoR<up>Hsp70Ba.PS</up>
TCL|cosmid
TFT|lambda cos sites (3)
|bacterial selection -> ampR
|chimeric gene -> Hsp70Ba | <fbsym>FBgn0014449==Tn\neoR</fbsym>
|reporter cassette (-ATG) | <fbsym>FBal0040953==Ecol\lacZ[C4]</fbsym>
|selectable marker | <fbsym>neoR<up>Hsp70Ba.PS</up></fbsym>
TPU|cloning vector | cosmid
|cloning vector | reporter
|cloning vector | transposon | P-element
CC|construct
|One of a series of cosmid P-transformation vectors with a <fbsym>neoR</fbsym> selectable
| marker and a <fbsym>lacZ</fbsym> reporter cassette; differ in cloning sites preceding
| the reporter cassette. KpnI cloning site present in <fbsym>FBmc0000354==cosP{neo&bgr;-c}</fbsym> only.
PRG|in vitro progenitor <fbsym>FBmc0000352==cosP{neo&bgr;-gal}</fbsym>
DEC|transposon descendant <fbsym>FBtp0001053==P{neo&bgr;-b}</fbsym>
}

REFDSR
{
RDID|FBrf0087820
|Thummel
|1996.8.8
TFT|general promoter | Hsp70Bb<up>+</up>
|inducible promoter | Hsp70Bb<up>+</up>
}

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
CS|In front of <fbsym>lacZ</fbsym> reporter cassette: SalI <up>KpnI</up> XbaI BamHI SmaI
}

REF
{
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0048083
|Thummel et al.
|1988
REFM|FBrf0053791
|Irvine et al.
|1991
REFM|FBrf0087820
|Thummel
|1996.8.8
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000354	CCL 1 -	SGTP 1 composite	SYM 1 cosP{neo&bgr;-c}	DBA 1 -	REF 1 5	DT 1 7 May 1999	RESZ 2835
ID|FBmc0000354
SYM|cosP{neo&bgr;-c}
SYN|cPnbetac
TRL|~13058
DT|7 May 1999
|21 Mar 1997
SGTP|composite
SEG|1 + <fbsym>FBms0000249==cosPneo_P5'(P5',R1)</fbsym>
|2 + <fbsym>FBms0003596==001236</fbsym>
|3 - <fbsym>FBms0000693==lacZ::SV40_4.2(-P)</fbsym>
|4 - <fbsym>FBms0003595==001237</fbsym>
|5 + <fbsym>FBms0000599==Car1_P3'(H3,P3')</fbsym>
|6 + <fbsym>FBms0003684==cosneo&bgr;-P</fbsym>
ASCO|<fbsym>FBmc0000353==cosP{neo&bgr;-b}</fbsym>
|<fbsym>FBmc0000354==cosP{neo&bgr;-c}</fbsym>
|<fbsym>FBmc0000352==cosP{neo&bgr;-gal}</fbsym>
ASGN|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>
|<fbsym>FBgn0014449==Tn\neoR</fbsym>
MMP|FBmc0000354
RSQ|FBmc0000354

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
ASAL|<fbsym>FBal0042319==Tn\neoR<up>Hsp70Bb.PS</up></fbsym>
TFT|lambda cos sites (3)
|allows selection with G418
|bacterial selection | ampR
|chimeric gene | Hsp70Bb::neoR
|chimeric gene | Hsp70Bb::<fbsym>FBgn0014449==Tn\neoR</fbsym>
|general promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|selectable marker | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|inducible promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|reporter cassette (-ATG) | lacZ<up>C4</up>
|selectable marker | neoR<up>Hsp70Bb.PS</up>
}

REFDSR
{
RDID|FBrf0048083
|Thummel et al.
|1988
ASAL|<fbsym>FBal0040953==Ecol\lacZ<up>C4</up></fbsym>
}

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
CS|In front of <fbsym>lacZ</fbsym> reporter cassette: SalI <up>KpnI</up> XbaI BamHI SmaI
SYN|cPnbetac
ASAL|neoR<up>Hsp70Ba.PS</up>
TCL|cosmid
TFT|lambda cos sites (3)
|bacterial selection -> ampR
|chimeric gene -> Hsp70Ba | <fbsym>FBgn0014449==Tn\neoR</fbsym>
|reporter cassette (-ATG) | <fbsym>FBal0040953==Ecol\lacZ[C4]</fbsym>
|selectable marker | <fbsym>neoR<up>Hsp70Ba.PS</up></fbsym>
TPU|cloning vector | cosmid
|cloning vector | reporter
|cloning vector | transposon | P-element
CC|construct
|One of a series of cosmid P-transformation vectors with a <fbsym>neoR</fbsym> selectable
| marker and a <fbsym>lacZ</fbsym> reporter cassette; differ in cloning sites preceding
| the reporter cassette. KpnI cloning site present in <fbsym>FBmc0000354==cosP{neo&bgr;-c}</fbsym> only.
PRG|in vitro progenitor <fbsym>FBmc0000352==cosP{neo&bgr;-gal}</fbsym>
DEC|in vitro descendant <fbsym>FBmc0000355==cosP{w&bgr;-gal}</fbsym>
|transposon descendant <fbsym>FBtp0001054==P{neo&bgr;-c}</fbsym>
}

REFDSR
{
RDID|FBrf0087820
|Thummel
|1996.8.8
TFT|general promoter | Hsp70Bb<up>+</up>
|inducible promoter | Hsp70Bb<up>+</up>
}

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
CS|In front of <fbsym>lacZ</fbsym> reporter cassette: SalI <up>KpnI</up> XbaI BamHI SmaI
}

REF
{
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0048083
|Thummel et al.
|1988
REFM|FBrf0053791
|Irvine et al.
|1991
REFM|FBrf0087820
|Thummel
|1996.8.8
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000352	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{neo&bgr;-gal}	DBA 1 -	REF 1 2	DT 1 7 May 1999	RESZ 2329
ID|FBmc0000352
SYM|cosP{neo&bgr;-gal}
SYN|cosPneobeta-gal
DT|7 May 1999
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000353==cosP{neo&bgr;-b}</fbsym>
|<fbsym>FBmc0000354==cosP{neo&bgr;-c}</fbsym>
|<fbsym>FBmc0000352==cosP{neo&bgr;-gal}</fbsym>
ASGN|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>
|<fbsym>FBgn0014449==Tn\neoR</fbsym>
MMP|FBmc0000352

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
ASAL|<fbsym>FBal0040953==Ecol\lacZ<up>C4</up></fbsym>
|<fbsym>FBal0042319==Tn\neoR<up>Hsp70Bb.PS</up></fbsym>
TCL|cosmid
TFT|allows selection with G418
|bacterial selection | ampR
|chimeric gene | Hsp70Bb::neoR
|reporter cassette (-ATG) | lacZ<up>C4</up>
|selectable marker | neoR<up>Hsp70Bb.PS</up>
TPU|cloning vector | cosmid
|cloning vector | reporter
|cloning vector | transposon | P-element
CC|construct
|One of a series of cosmid P-transformation vectors with a <fbsym>neoR</fbsym> selectable
| marker and a <fbsym>lacZ</fbsym> reporter cassette; differ in cloning sites preceding
| the reporter cassette.
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|in vitro progenitor <fbsym>FBmc0000219==pP{C4&bgr;gal}</fbsym>
DEC|in vitro descendant <fbsym>FBtp0001100==P{neo&bgr;-gal}</fbsym>
}

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
CS|In front of <fbsym>lacZ</fbsym> reporter cassette: BamHI SmaI
SYN|cosPneobeta-gal
ASAL|neoR<up>Hsp70Ba.PS</up>
TFT|lambda cos sites (3)
|bacterial selection -> ampR
|chimeric gene -> Hsp70Ba | <fbsym>FBgn0014449==Tn\neoR</fbsym>
|reporter cassette (-ATG) | <fbsym>FBal0040953==Ecol\lacZ[C4]</fbsym>
|selectable marker | <fbsym>neoR<up>Hsp70Ba.PS</up></fbsym>
TPU|cloning vector | cosmid
|cloning vector | transposon | P-element
CC|construct
|One of a series of cosmid P-transformation vectors with a <fbsym>neoR</fbsym> selectable
| marker and a <fbsym>lacZ</fbsym> reporter cassette; differ in cloning sites preceding
| the reporter cassette.
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|in vitro progenitor <fbsym>FBmc0000219==pP{C4&bgr;gal}</fbsym>
DEC|in vitro descendant <fbsym>FBmc0000353==cosP{neo&bgr;-b}</fbsym>
|in vitro descendant <fbsym>FBmc0000354==cosP{neo&bgr;-c}</fbsym>
|in vitro descendant <fbsym>FBmc0000359==cosP{5UZ-neo}</fbsym>
}

REF
{
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0053791
|Irvine et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000913	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{neoR.A}	DBA 1 -	REF 1 4	DT 1 8 Apr 1997	RESZ 1680
ID|FBmc0000913
SYM|cosP{neoR.A}
SYN|cosA
|cosP{A}
TRL|~13380
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 + <fbsym>FBms0000249==cosPneo_P5'(P5',R1)</fbsym>
|2 + <fbsym>FBms0000086==M13mp10mcs</fbsym>
|3 + <fbsym>FBms0000599==Car1_P3'(H3,P3')</fbsym>
|4 + <fbsym>FBms0003683==cosneoRA-P</fbsym>
ASCO|<fbsym>FBmc0000913==cosP{neoR.A}</fbsym>
|<fbsym>FBmc0000136==cosP{neoR}</fbsym>
ASGN|<fbsym>FBgn0014449==Tn\neoR</fbsym>
MMP|FBmc0000913
RSQ|FBmc0000913

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
ASAL|<fbsym>FBal0042319==Tn\neoR<up>Hsp70Bb.PS</up></fbsym>
TFT|lambda cos sites (3)
|allows selection with G418
|chimeric gene | Hsp70Bb::<fbsym>FBgn0014449==Tn\neoR</fbsym>
|general promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|selectable marker | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|inducible promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
}

REFDSR
{
RDID|FBrf0049623
|Rushlow et al.
|1989
CS|BamHI
SYN|cosA
TCL|cosmid
TFT|bacterial selection | kanR
TPU|cloning vector | cosmid
CC|construct
|Allows cloning of smaller fragments (<30kb) than <fbsym>FBmc0000136==cosP{neoR}</fbsym>.
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
DEC|in vitro descendant <fbsym>FBmc0000912==cosP{cosh}</fbsym>
|transposon descendant <fbsym>FBtp0001052==P{neoR.A}</fbsym>
}

REFDSR
{
RDID|FBrf0087820
|Thummel
|1996.8.8
TFT|general promoter | Hsp70Bb<up>+</up>
|inducible promoter | Hsp70Bb<up>+</up>
}

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
CS|BamHI
}

REF
{
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0049623
|Rushlow et al.
|1989
REFM|FBrf0087820
|Thummel
|1996.8.8
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000136	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{neoR}	DBA 1 -	REF 1 10	DT 1 8 Apr 1997	RESZ 2711
ID|FBmc0000136
SYM|cosP{neoR}
SYN|cosPneo
TRL|~8874
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 + <fbsym>FBms0000247==cosPneo-mcs</fbsym>
|2 + <fbsym>FBms0000086==M13mp10mcs</fbsym>
ASGN|<fbsym>FBgn0014449==Tn\neoR</fbsym>
MMP|FBmc0000136
RSQ|FBmc0000136

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
ENL|HindIII
ENR|HindIII (inclusive)
CS|XbaI BamHI SmaI SstI EcoRI
SYN|cosPneo
ASAL|<fbsym>FBal0042319==Tn\neoR<up>Hsp70Bb.PS</up></fbsym>
TCL|cosmid
TFT|lambda cos sites (3)
|allows selection with G418
|bacterial selection | ampR
|bacterial selection | kanR
|chimeric gene | Hsp70Bb::<fbsym>FBgn0014449==Tn\neoR</fbsym>
|general promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|selectable marker | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|inducible promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
TPU|cloning vector | cosmid
|cloning vector | transposon | P-element
PRG|in vitro progenitor <fbsym>FBmc0000134==pP{Ca4hsneo}</fbsym>
|in vitro progenitor <fbsym>FBmc0000135==cosP</fbsym>
DEC|in vitro descendant <fbsym>FBmc0000352==cosP{neo&bgr;-gal}</fbsym>
|transposon descendant <fbsym>FBtp0000080==P{neoR}</fbsym>
}

REFDSR
{
RDID|FBrf0045759
|Ish-Horowicz and Pinchin
|1987
DEC|in vitro descendant <fbsym>FBmc0000538==cosP{HSH3}</fbsym>
|in vitro descendant <fbsym>FBmc0000539==cosP{HSH4}</fbsym>
}

REFDSR
{
RDID|FBrf0047305
|Hafen et al.
|1987
DEC|in vitro descendant <fbsym>FBmc0000599==cosP{sev-15}</fbsym>
}

REFDSR
{
RDID|FBrf0048075
|Speek et al.
|1988
DEC|in vitro descendant <fbsym>FBmc0001078==cosP{smart2}</fbsym>
}

REFDSR
{
RDID|FBrf0048102
|Boulet and Scott
|1988
DEC|in vitro descendant <fbsym>FBmc0001091==pP{C4hsneo-lacZ}</fbsym>
}

REFDSR
{
RDID|FBrf0049623
|Rushlow et al.
|1989
DEC|in vitro descendant <fbsym>FBmc0000913==cosP{neoR.A}</fbsym>
}

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
DEC|in vitro descendant <fbsym>FBmc0000352==cosP{neo&bgr;-gal}</fbsym>
}

REFDSR
{
RDID|FBrf0054058
|Dreesen et al.
|1991
DEC|in vitro descendant <fbsym>FBmc0000363==cosP{bw<up>+</up>1111}</fbsym>
}

REFDSR
{
RDID|FBrf0075322
|Pirrotta
|1994
DEC|in vitro descendant <fbsym>FBmc0000234==cosP{cosPer}</fbsym>
}

REFDSR
{
RDID|FBrf0087820
|Thummel
|1996.8.8
TFT|general promoter | Hsp70Bb<up>+</up>
|inducible promoter | Hsp70Bb<up>+</up>
}

REF
{
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0045759
|Ish-Horowicz and Pinchin
|1987
REFM|FBrf0047305
|Hafen et al.
|1987
REFM|FBrf0048075
|Speek et al.
|1988
REFM|FBrf0048102
|Boulet and Scott
|1988
REFM|FBrf0049623
|Rushlow et al.
|1989
REFM|FBrf0053791
|Irvine et al.
|1991
REFM|FBrf0054058
|Dreesen et al.
|1991
REFM|FBrf0075322
|Pirrotta
|1994
REFM|FBrf0087820
|Thummel
|1996.8.8
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001361	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{NT1B1}	DBA 1 -	REF 1 1	DT 1 8 Jun 1998	RESZ 592
ID|FBmc0001361
SYM|cosP{NT1B1}
DT|8 Jun 1998
|9 Apr 1998
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0011706==rpr</fbsym>

REFDSR
{
RDID|FBrf0074787
|White et al.
|1994
ASAL|<fbsym>FBal0044322==rpr<up>NT1B1</up></fbsym>
TCL|cosmid
TFT|partial rescue | <fbsym>FBal0044322==rpr[NT1B1]</fbsym>
TPU|characterization | <fbsym>FBgn0011706==rpr</fbsym>
PRG|in vitro progenitor <fbsym>FBmc0000234==cosP{cosPer}</fbsym>
|in vitro progenitor: <fbsym>FBgn0011706==rpr</fbsym> genomic sequences
DEC|transposon descendant <fbsym>FBtp0001386==P{NT1B1}</fbsym>
}

REF
{
REFM|FBrf0074787
|White et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000387	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{ovoD1-S23-1}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 626
ID|FBmc0000387
SYM|cosP{ovoD1-S23-1}
SYN|Cos-P[ovo<up>D1</up>]<up>S23-1</up>
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003028==ovo</fbsym>

REFDSR
{
RDID|FBrf0068449
|Chou et al.
|1993
SYN|Cos-P[ovo<up>D1</up>]<up>S23-1</up>
ASAL|<fbsym>FBal0039715==ovo<up>D1.S23-1</up></fbsym>
TCL|cosmid
TPU|recombinant cloning construct | <fbsym>FBgn0003028==ovo</fbsym>
PRG|in vitro progenitor <fbsym>FBmc0000234==cosP{cosPer}</fbsym>
|in vitro progenitor: ovo<up>D1</up> genomic DNA (FBrf0068449)
DEC|in vitro descendant <fbsym>FBmc0000388==pP{ovoD1-18}</fbsym>
}

REF
{
REFM|FBrf0068449
|Chou et al.
|1993
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000599	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{sev-15}	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 698
ID|FBmc0000599
SYM|cosP{sev-15}
SYN|cosP{sev15}
DT|13 May 1999
|21 Mar 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003366==sev</fbsym>

REFDSR
{
RDID|FBrf0047305
|Hafen et al.
|1987
ASAL|neoR<up>Hsp70Bb.PS</up>
|<fbsym>FBal0039883==sev<up>+t16.3</up></fbsym>
TCL|cosmid
TFT|complete rescue | <fbsym>FBal0039883==sev[+t16.3]</fbsym>
|selectable marker | <fbsym>neoR<up>Hsp70Bb.PS</up></fbsym>
TPU|characterization | <fbsym>FBgn0003366==sev</fbsym>
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|in vitro progenitor: <fbsym>FBgn0003366==sev</fbsym> genomic sequences
DEC|transposon descendant <fbsym>FBtp0000023==P{sev-15}</fbsym>
}

REF
{
REFM|FBrf0047305
|Hafen et al.
|1987
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001078	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{smart2}	DBA 1 -	REF 1 4	DT 1 7 Jun 1997	RESZ 1922
ID|FBmc0001078
SYM|cosP{smart2}
SYN|smart2
TRL|~8503
DT|7 Jun 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 + <fbsym>FBms0000065==Car1_P5'(P5',R1)</fbsym>
|2 + <fbsym>FBms0000239==<up>EcoRI</up>ef/<up>SmaI</up>b</fbsym>
|3 - <fbsym>FBms0000240==Hsp70::neo_1.4(Xb,Sm)</fbsym>
|4 + <fbsym>FBms0000241==001040</fbsym>
|5 + <fbsym>FBms0002872==001252</fbsym>
|6 + <fbsym>FBms0002873==Lorist2.cs</fbsym>
|7 + <fbsym>FBms0003299==EcoRI</fbsym>
|8 + <fbsym>FBms0002874==&pgr;25.7_P3'</fbsym>
|9 + <fbsym>FBms0003685==cos.smart2-P</fbsym>
ASGN|<fbsym>FBgn0014449==Tn\neoR</fbsym>
MMP|FBmc0001078
RSQ|FBmc0001078

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
ASAL|<fbsym>FBal0042319==Tn\neoR<up>Hsp70Bb.PS</up></fbsym>
TFT|allows selection with G418
|chimeric gene | Hsp70Bb::<fbsym>FBgn0014449==Tn\neoR</fbsym>
|general promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|selectable marker | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
|inducible promoter | <fbsym>FBal0042319==Tn\neoR[Hsp70Bb.PS]</fbsym>
}

REFDSR
{
RDID|FBrf0048075
|Speek et al.
|1988
CS|BamHI
SYN|smart2
TCL|cosmid
TFT|bacterial selection | Tn10\tetR
TPU|cloning vector | cosmid
|cloning vector | transposon | P-element
CC|report
|CAUTION: Extent of several segments uncertain (see map).
PRG|in vitro progenitor <fbsym>FBmc0000136==cosP{neoR}</fbsym>
|in vitro progenitor: Lorist2 (sequence entry X98409 | 1403131 | 29 Jul 1996)
|in vitro progenitor: pRE3 (source of tetR; Moreau et al., 1981, Nucl. Acids Res. 9: 6047-6068)
DEC|transposon descendant <fbsym>FBtp0001212==P{smart2}</fbsym>
}

REFDSR
{
RDID|FBrf0087820
|Thummel
|1996.8.8
TFT|general promoter | Hsp70Bb<up>+</up>
|inducible promoter | Hsp70Bb<up>+</up>
}

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
CS|BamHI
}

REF
{
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0048075
|Speek et al.
|1988
REFM|FBrf0087820
|Thummel
|1996.8.8
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000355	CCL 1 constr	SGTP 1 composite	SYM 1 cosP{w&bgr;-gal}	DBA 1 -	REF 1 5	DT 1 7 May 1999	RESZ 2452
ID|FBmc0000355
SYM|cosP{w&bgr;-gal}
SYN|cPwbeta
TRL|~15738
DT|7 May 1999
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 + <fbsym>FBms0000599==Car1_P3'(H3,P3')</fbsym>
|2 + <fbsym>FBms0000096==w<up>+</up>car6.1_3'</fbsym>
|3 + <fbsym>FBms0003591==pUC8::cos4</fbsym>
|4 + <fbsym>FBms0002890==<up>SalI</up>ef</fbsym>
|5 + <fbsym>FBms0000008==w<up>+</up>0.5(S,6.1)</fbsym>
|6 + <fbsym>FBms0000065==Car1_P5'(P5',R1)</fbsym>
|7 + <fbsym>FBms0000726==001105</fbsym>
|8 - <fbsym>FBms0000515==w<up>+mC</up>4.1(Sp)</fbsym>
|9 + <fbsym>FBms0000517==001068</fbsym>
|10 + <fbsym>FBms0003596==001236</fbsym>
|11 - <fbsym>FBms0000693==lacZ::SV40_4.2(-P)</fbsym>
|12 - <fbsym>FBms0003597==001238</fbsym>
ASGN|<fbsym>FBgn0003996==w</fbsym>
|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>
MMP|FBmc0000355
RSQ|FBmc0000355

REFDSR
{
RDID|FBrf0042419
|Steller and Pirrotta
|1985
TFT|lambda cos sites (3)
|bacterial selection | ampR
|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|reporter cassette (-ATG) | lacZ<up>C4</up>
}

REFDSR
{
RDID|FBrf0048083
|Thummel et al.
|1988
ASAL|<fbsym>FBal0040953==Ecol\lacZ<up>C4</up></fbsym>
}

REFDSR
{
RDID|FBrf0048982
|Pirrotta
|1988
ASAL|<fbsym>FBal0028610==w<up>+mC</up></fbsym>
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|partial rescue | mini-gene::<fbsym>FBal0028610==w[+mC]</fbsym>
}

REFDSR
{
RDID|FBrf0051386
|Nambu et al.
|1990
DEC|transposon descendant <fbsym>FBtp0003497==P{7.8sim/lacZ}</fbsym>
}

REFDSR
{
RDID|FBrf0053791
|Irvine et al.
|1991
ENL|HindIII
ENR|HindIII (inclusive)
CS|In front of <fbsym>lacZ</fbsym> reporter cassette: KpnI XbaI BamHI
SYN|cPwbeta
TCL|cosmid
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|bacterial selection -> ampR
|reporter cassette (-ATG) | <fbsym>FBal0040953==Ecol\lacZ[C4]</fbsym>
TPU|cloning vector | cosmid
|cloning vector | reporter
|cloning vector | transposon | P-element
PRG|in vitro progenitor <fbsym>FBmc0000234==cosP{cosPer}</fbsym>
|in vitro progenitor <fbsym>FBmc0000354==cosP{neo&bgr;-c}</fbsym>
DEC|in vitro descendant <fbsym>FBmc0000356==cosP{35UZ}</fbsym>
|in vitro descendant <fbsym>FBmc0000357==cosP{22UZ}</fbsym>
|in vitro descendant <fbsym>FBmc0000358==cosP{5UZ}</fbsym>
|transposon descendant <fbsym>FBtp0001055==P{w&bgr;-gal}</fbsym>
}

REF
{
REFM|FBrf0042419
|Steller and Pirrotta
|1985
REFM|FBrf0048083
|Thummel et al.
|1988
REFM|FBrf0048982
|Pirrotta
|1988
REFM|FBrf0051386
|Nambu et al.
|1990
REFM|FBrf0053791
|Irvine et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002745	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-EnGypGypOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 448
ID|FBmc0002745
SYM|eFRT-EnGypGypOff
SYN|EnGypGypOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-EnGypGypOff
|EnGypGypOff
ASAL|<fbsym>FBal0120287==w<up>EnGypGypOff</up></fbsym>
TCL|episome
TPU|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013530==P{FRT(EnGypGypOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002743	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-EnGypOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 430
ID|FBmc0002743
SYM|eFRT-EnGypOff
SYN|EnGypOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-EnGypOff
|EnGypOff
ASAL|<fbsym>FBal0120285==w<up>EnGypOff</up></fbsym>
TCL|episome
TPU|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013529==P{FRT(EnGypOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002740	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-EnOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 455
ID|FBmc0002740
SYM|eFRT-EnOff
SYN|EnOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-EnOff
|EnOff
ASAL|<fbsym>FBal0120283==w<up>EnOff</up></fbsym>
TCL|episome
TPU|control construct
|characterization | scs
|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013528==P{FRT(EnOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002749	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-EnScsOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 419
ID|FBmc0002749
SYM|eFRT-EnScsOff
SYN|EnScsOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-EnScsOff
|EnScsOff
ASAL|<fbsym>FBal0120281==w<up>EnScsOff</up></fbsym>
TCL|episome
TPU|characterization | scs
PRG|progenitor <fbsym>FBtp0013527==P{FRT(EnScsOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002741	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-EnYellowOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 491
ID|FBmc0002741
SYM|eFRT-EnYellowOff
SYN|EnYellowOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-EnYellowOff
|EnYellowOff
ASAL|<fbsym>FBal0120279==w<up>EnYellowOff</up></fbsym>
TCL|episome
TPU|control construct
|characterization | scs
|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013516==P{FRT(EnYellowOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001431	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-GFP.X97	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 877
ID|FBmc0001431
SYM|eFRT-GFP.X97
DT|13 May 1999
|6 Jul 1998
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>
|<fbsym>FBgn0014446==Avic\GFP</fbsym>

REFDSR
{
RDID|FBrf0098247
|Golic et al.
|1997
ENL|FRT (inclusive)
ENR|FRT (exclusive)
ASAL|<fbsym>FBal0083443==Avic\GFP<up>hs.P</up></fbsym>
|<fbsym>FBal0081798==w<up>X97.hs</up></fbsym>
TCL|episome
TFT|general promoter | GFP<up>hs.P</up>
|inducible promoter | GFP<up>hs.P</up>
|chimeric gene | FRT::w::Hsp70::GFP::w::FRT
|removable cassette | FRT-w<up>RS3r.stop</up>,GFP,w<up>RS5r</up>-FRT
TPU|characterization | FLP1/FRT system
CC|construct
|Generated by action of <fbsym>FLP1</fbsym> recombinase from <fbsym>FBtp0001547==P{FRT(GFP.X97)}</fbsym>.
|report
|See report for <fbsym>FBgn0015220==Scer\FRT</fbsym>.
PRG|progenitor <fbsym>FBtp0001547==P{FRT(GFP.X97)}</fbsym>
}

REF
{
REFM|FBrf0098247
|Golic et al.
|1997
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002744	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-GypEnGypOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 448
ID|FBmc0002744
SYM|eFRT-GypEnGypOff
SYN|GypEnGypOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-GypEnGypOff
|GypEnGypOff
ASAL|<fbsym>FBal0120277==w<up>GypEnGypOff</up></fbsym>
TCL|episome
TPU|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013526==P{FRT(GypEnGypOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002742	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-GypEnOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 430
ID|FBmc0002742
SYM|eFRT-GypEnOff
SYN|GypEnOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-GypEnOff
|GypEnOff
ASAL|<fbsym>FBal0120275==w<up>GypEnOff</up></fbsym>
TCL|episome
TPU|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013525==P{FRT(GypEnOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002746	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-GypGypEnOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 448
ID|FBmc0002746
SYM|eFRT-GypGypEnOff
SYN|GypGypEnOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-GypGypEnOff
|GypGypEnOff
ASAL|<fbsym>FBal0120273==w<up>GypGypEnOff</up></fbsym>
TCL|episome
TPU|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013524==P{FRT(GypGypEnOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002747	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-GypYellowEnGypOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 484
ID|FBmc0002747
SYM|eFRT-GypYellowEnGypOff
SYN|GypYellowEnGypOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-GypYellowEnGypOff
|GypYellowEnGypOff
ASAL|<fbsym>FBal0120271==w<up>GypYellowEnGypOff</up></fbsym>
TCL|episome
TPU|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013523==P{FRT(GypYellowEnGypOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002739	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-mini-Off	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 473
ID|FBmc0002739
SYM|eFRT-mini-Off
SYN|mini-Off
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-mini-Off
|mini-Off
ASAL|<fbsym>FBal0120261==w<up>mini-Off</up></fbsym>
TCL|episome
TPU|control construct
|characterization | scs
|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013518==P{FRT(mini-Off)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002751	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-Scs-mini-Off	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 443
ID|FBmc0002751
SYM|eFRT-Scs-mini-Off
SYN|Scs-mini-Off
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-Scs-mini-Off
|Scs-mini-Off
ASAL|<fbsym>FBal0120269==w<up>Scs-mini-Off</up></fbsym>
TCL|episome
TPU|characterization | scs
PRG|progenitor <fbsym>FBtp0013522==P{FRT(Scs-mini-Off)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002752	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-ScsEnGypOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 472
ID|FBmc0002752
SYM|eFRT-ScsEnGypOff
SYN|ScsEnGypOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-ScsEnGypOff
|ScsEnGypOff
ASAL|<fbsym>FBal0120267==w<up>ScsEnGypOff</up></fbsym>
TCL|episome
TPU|characterization | scs
|characterization | gypsy\su(Hw)BR
PRG|progenitor <fbsym>FBtp0013521==P{FRT(ScsEnGypOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002748	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-ScsEnOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 419
ID|FBmc0002748
SYM|eFRT-ScsEnOff
SYN|ScsEnOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-ScsEnOff
|ScsEnOff
ASAL|<fbsym>FBal0120265==w<up>ScsEnOff</up></fbsym>
TCL|episome
TPU|characterization | scs
PRG|progenitor <fbsym>FBtp0013520==P{FRT(ScsEnOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002750	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-ScsEnScsOff	DBA 1 -	REF 1 1	DT 1 20 Feb 2001	RESZ 437
ID|FBmc0002750
SYM|eFRT-ScsEnScsOff
SYN|ScsEnScsOff
DT|20 Feb 2001
|20 Feb 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0132057
|Parnell and Geyer
|2000
SYN|eFRT-ScsEnScsOff
|ScsEnScsOff
ASAL|<fbsym>FBal0120263==w<up>ScsEnScsOff</up></fbsym>
TCL|episome
TPU|characterization | scs
PRG|progenitor <fbsym>FBtp0013519==P{FRT(ScsEnScsOff)}</fbsym>
}

REF
{
REFM|FBrf0132057
|Parnell and Geyer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001467	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-w.G	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 753
ID|FBmc0001467
SYM|eFRT-w.G
DT|13 May 1999
|6 Nov 1998
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0090428
|Ahmad and Golic
|1996
ENL|FRT (inclusive)
ENR|FRT (exclusive)
ASAL|<fbsym>FBal0028611==w<up>+mW.hs</up></fbsym>
TCL|episome
TFT|selectable marker | <fbsym>FBal0028611==w[+mW.hs]</fbsym>
|removable cassette | FRT-w<up>+mW.hs</up>-FRT
TPU|characterization | Scer\FLP1
CC|construct
|Generated by action of <fbsym>FLP1</fbsym> recombinase from
| <fbsym>FBtp0000268==P{FRT(w<up>hs</up>)}</fbsym>. Carries a functional mini-white marker.
|report
|See report for <fbsym>FBgn0015220==Scer\FRT</fbsym>.
PRG|progenitor <fbsym>FBtp0000268==P{FRT(w<up>hs</up>)}</fbsym>
}

REF
{
REFM|FBrf0090428
|Ahmad and Golic
|1996
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001465	CCL 1 constr	SGTP 1 composite	SYM 1 eFRT-w.X96	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 780
ID|FBmc0001465
SYM|eFRT-w.X96
DT|13 May 1999
|6 Nov 1998
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003996==w</fbsym>

REFDSR
{
RDID|FBrf0090428
|Ahmad and Golic
|1996
ENL|FRT (inclusive)
ENR|FRT (exclusive)
ASAL|<fbsym>FBal0065637==w<up>+mW.Scer\FRT.hs2</up></fbsym>
TCL|episome
TFT|selectable marker | w<up>+mW.FRT.hs2</up>
|removable cassette | FRT-w<up>RS3r</up>,w<up>RS5r</up>-FRT
TPU|characterization | Scer\FLP1
|characterization | FLP1/FRT system
CC|construct
|Generated by action of <fbsym>FLP1</fbsym> recombinase from <fbsym>FBtp0001601==P{FRT(X96)}</fbsym>. Carries a
| functional mini-white marker.
|report
|See report for <fbsym>FBgn0015220==Scer\FRT</fbsym>.
PRG|progenitor <fbsym>FBtp0001601==P{FRT(X96)}</fbsym>
}

REF
{
REFM|FBrf0090428
|Ahmad and Golic
|1996
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001379	CCL 1 constr	SGTP 1 composite	SYM 1 Lorist6	DBA 1 -	REF 1 3	DT 1 13 May 1999	RESZ 661
ID|FBmc0001379
SYM|Lorist6
SYN|6
|Lorist6
DT|13 May 1999
|21 Mar 1997
CCL|constr
SGTP|composite
ID2|FBmc0000809
MMP|FBmc0001379

REFDSR
{
RDID|FBrf0080225
|Madueno et al.
|1995
TCL|cosmid
TPU|cloning vector | cosmid
CC|construct
|Cosmid vector used for STS mapping by the European Drosophila Mapping
| Project. See Gibson et al., 1987, Gene, 53: 283-286.
|report
|Sequence available by ftp at genome.wustl.edu (log-in "anonymous") in file /pub/gsc1/sequence/vector/lorist6.seq.
}

REFDSR
{
RDID|FBrf0172294
|Matthews
|1996.6.12
SYN|Lorist6
}

REF
{
REFM|FBrf0080225
|Madueno et al.
|1995
REFM|FBrf0105495
|FlyBase
|1992-
REFM|FBrf0172294
|Matthews
|1996.6.12
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002788	CCL 1 constr	SGTP 1 composite	SYM 1 p0-CAT	DBA 1 -	REF 1 2	DT 1 26 Mar 2002	RESZ 1024
ID|FBmc0002788
SYM|p0-CAT
TRL|~4006
DT|26 Mar 2002
|21 Mar 1997
CCL|constr
SGTP|composite
ID2|FBtp0001083
SEG|1 - <fbsym>FBms0003649==pML2(S,R1)</fbsym>
|2 + <fbsym>FBms0003647==001198</fbsym>
|3 + <fbsym>FBms0000677==CAT::SV40_1.6(-P)</fbsym>
|4 + <fbsym>FBms0003648==001197</fbsym>
ASGN|<fbsym>FBgn0014442==Ecol\CAT</fbsym>

REFDSR
{
RDID|FBrf0048083
|Thummel et al.
|1988
ASAL|<fbsym>FBal0040258==Ecol\CAT<up>C4</up></fbsym>
}

REFDSR
{
RDID|FBrf0048506
|Martin and Jarry
|1988
ENL|EcoRI
ENR|EcoRI (inclusive)
CS|In front of <fbsym>CAT</fbsym> reporter gene: SalI BglII HindIII
TCL|plasmid
|transposon | P-element
TFT|bacterial selection | ampR
|reporter cassette (+ATG) |                                                               <fbsym>FBal0040258==Ecol\CAT<up>C4</up></fbsym>
TPU|cloning vector | reporter
|cloning vector | cell culture assay
PRG|in vitro progenitor: pC&agr;-CAT  (Herbomel et al., 1984, Cell 39: 653-662)
}

REF
{
REFM|FBrf0048083
|Thummel et al.
|1988
REFM|FBrf0048506
|Martin and Jarry
|1988
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000731	CCL 1 constr	SGTP 1 composite	SYM 1 p3CAT	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 841
ID|FBmc0000731
SYM|p3CAT
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000731==p3CAT</fbsym>
|<fbsym>FBmc0000732==ptwi(a)-CAT</fbsym>
|<fbsym>FBmc0000730==ptwi-CAT</fbsym>
ASGN|<fbsym>FBgn0014442==Ecol\CAT</fbsym>

REFDSR
{
RDID|FBrf0053349
|Thisse et al.
|1991
ASAL|<fbsym>FBal0040434==Ecol\CAT<up>twi.3000P</up></fbsym>
TCL|plasmid
TFT|reporter gene | <fbsym>FBal0040434==Ecol\CAT[twi.3000P]</fbsym>
|chimeric gene | twi::Ecol\CAT::Simian virus 40
TPU|reporter construct | twi
|cell culture assay | S2 cells
CC|construct
|One of a series of <fbsym>FBgn0003900==twi</fbsym>-<fbsym>CAT</fbsym> fusion constructs used to
| characterize <fbsym>FBgn0003900==twi</fbsym> 5' regulatory elements and their interactions with the
| dorsal protein, using a cell culture assay.
}

REF
{
REFM|FBrf0053349
|Thisse et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001438	CCL 1 constr	SGTP 1 composite	SYM 1 p7xEcRE-lacZ	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 1088
ID|FBmc0001438
SYM|p7xEcRE-lacZ
DT|13 May 1999
|3 Aug 1998
CCL|constr
SGTP|composite
ASTR|<fbsym>FBtr0007185==Ecol\lacZ<up>Adh.EcRE</up>RA</fbsym>
ASGN|<fbsym>FBgn0003996==w</fbsym>
|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>

REFDSR
{
RDID|FBrf0102541
|Munroe et al.
|1998.5.12
ASAL|<fbsym>FBal0057023==Ecol\lacZ<up>Adh.EcRE</up></fbsym>
|<fbsym>FBal0028610==w<up>+mC</up></fbsym>
TCL|plasmid
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|reporter gene | <fbsym>FBal0057023==Ecol\lacZ[Adh.EcRE]</fbsym>
|chimeric gene | 7xEcRE::Adh::<fbsym>FBgn0014447==Ecol\lacZ</fbsym>
TPU|reporter construct | Hsp27
|characterization | EcR | protein binding
|synthetic DNA element | EcR protein binding site(s)
CC|report
|P{EcRE.lacZ} carries a reporter gene consisting of 7 copies of a 30bp
| sequence from <fbsym>FBgn0001226==Hsp27</fbsym>, which includes the 23-bp EcRE region; these are
| followed by the Adh distal promoter; followed by the <fbsym>lacZ</fbsym> gene.
DEC|in vitro descendant <fbsym>FBmc0001437==pP{EcRE.lacZ}</fbsym>
}

REF
{
REFM|FBrf0102541
|Munroe et al.
|1998.5.12
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000073	CCL 1 constr	SGTP 1 -	SYM 1 pAc-&Dgr;zf	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 718
ID|FBmc0000073
SYM|pAc-&Dgr;zf
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
ASCO|<fbsym>FBmc0000073==pAc-&Dgr;zf</fbsym>
|<fbsym>FBmc0000072==pAc-esg</fbsym>
|<fbsym>FBmc0000815==pE2-CAT</fbsym>
|<fbsym>FBmc0000070==phs-sc</fbsym>
|<fbsym>FBmc0000071==phsp-da</fbsym>

REFDSR
{
RDID|FBrf0076506
|Fuse et al.
|1994
TPU|characterization | esg
|characterization | E2 box
|cell culture assay | S2 cells
|characterization | bHLH-protein binding
CC|construct
|Used in conjunction with <fbsym>FBmc0000815==pE2-CAT</fbsym> and other members of series to
| characterize bHLH-protein binding to E2 box sequences, assayed by
| subsequent transcription of <fbsym>CAT</fbsym> reporter gene.
}

REF
{
REFM|FBrf0076506
|Fuse et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000072	CCL 1 constr	SGTP 1 -	SYM 1 pAc-esg	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 714
ID|FBmc0000072
SYM|pAc-esg
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
ASCO|<fbsym>FBmc0000073==pAc-&Dgr;zf</fbsym>
|<fbsym>FBmc0000072==pAc-esg</fbsym>
|<fbsym>FBmc0000815==pE2-CAT</fbsym>
|<fbsym>FBmc0000070==phs-sc</fbsym>
|<fbsym>FBmc0000071==phsp-da</fbsym>

REFDSR
{
RDID|FBrf0076506
|Fuse et al.
|1994
TPU|characterization | esg
|characterization | E2 box
|cell culture assay | S2 cells
|characterization | bHLH-protein binding
CC|construct
|Used in conjunction with <fbsym>FBmc0000815==pE2-CAT</fbsym> and other members of series to
| characterize bHLH-protein binding to E2 box sequences, assayed by
| subsequent transcription of <fbsym>CAT</fbsym> reporter gene.
}

REF
{
REFM|FBrf0076506
|Fuse et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000751	CCL 1 constr	SGTP 1 composite	SYM 1 pACH	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 309
ID|FBmc0000751
SYM|pACH
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0053349
|Thisse et al.
|1991
TCL|plasmid
TFT|chimeric gene | Act5C::Ecol\lacZ
TPU|control construct
|cell culture assay
PRG|in vitro progenitor: pCH110 (Pharmacia)
}

REF
{
REFM|FBrf0053349
|Thisse et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002885	CCL 1 constr	SGTP 1 composite	SYM 1 pACW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 681
ID|FBmc0002885
SYM|pACW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pACW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBal0141352==T:Avic\GFP<up>ECFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002885==pACW</fbsym> and <fbsym>FBmc0002886==pAWC</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002930	CCL 1 constr	SGTP 1 composite	SYM 1 pAFHW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 635
ID|FBmc0002930
SYM|pAFHW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAFHW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBgn0015306==T:Ivir\HA1</fbsym>
|cloning vector | epitope tag | <fbsym>FBgn0015304==T:Zzzz\FLAG</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002934	CCL 1 constr	SGTP 1 composite	SYM 1 pAFMW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 635
ID|FBmc0002934
SYM|pAFMW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAFMW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBgn0015310==T:Hsap\MYC</fbsym>
|cloning vector | epitope tag | <fbsym>FBgn0015304==T:Zzzz\FLAG</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002925	CCL 1 constr	SGTP 1 composite	SYM 1 pAFW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 669
ID|FBmc0002925
SYM|pAFW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAFW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBgn0015304==T:Zzzz\FLAG</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002925==pAFW</fbsym> and <fbsym>FBmc0002926==pAWF</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002877	CCL 1 constr	SGTP 1 composite	SYM 1 pAGW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 681
ID|FBmc0002877
SYM|pAGW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAGW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBal0097096==T:Avic\GFP<up>EGFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002877==pAGW</fbsym> and <fbsym>FBmc0002878==pAWG</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002910	CCL 1 constr	SGTP 1 composite	SYM 1 pAHW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 668
ID|FBmc0002910
SYM|pAHW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAHW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBgn0015306==T:Ivir\HA1</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002909==pAWH</fbsym> and <fbsym>FBmc0002910==pAHW</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002917	CCL 1 constr	SGTP 1 composite	SYM 1 pAMW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 668
ID|FBmc0002917
SYM|pAMW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAMW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBgn0015310==T:Hsap\MYC</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002917==pAMW</fbsym> and <fbsym>FBmc0002918==pAWM</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002901	CCL 1 constr	SGTP 1 composite	SYM 1 pARW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 681
ID|FBmc0002901
SYM|pARW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pARW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBal0155770==T:Disc\RFP<up>mRFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002901==pARW</fbsym> and <fbsym>FBmc0002902==pAWR</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002893	CCL 1 constr	SGTP 1 composite	SYM 1 pAVW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 686
ID|FBmc0002893
SYM|pAVW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAVW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
|cloning vector | epitope tag | <fbsym>FBal0155771==T:Avic\GFP<up>YFP.Venus</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002893==pAVW</fbsym> and <fbsym>FBmc0002894==pAWV</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002938	CCL 1 constr	SGTP 1 composite	SYM 1 pAW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 488
ID|FBmc0002938
SYM|pAW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002886	CCL 1 constr	SGTP 1 composite	SYM 1 pAWC	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 681
ID|FBmc0002886
SYM|pAWC
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAWC
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBal0141352==T:Avic\GFP<up>ECFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002885==pACW</fbsym> and <fbsym>FBmc0002886==pAWC</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002926	CCL 1 constr	SGTP 1 composite	SYM 1 pAWF	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 669
ID|FBmc0002926
SYM|pAWF
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAWF
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBgn0015304==T:Zzzz\FLAG</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002925==pAFW</fbsym> and <fbsym>FBmc0002926==pAWF</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002878	CCL 1 constr	SGTP 1 composite	SYM 1 pAWG	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 681
ID|FBmc0002878
SYM|pAWG
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAWG
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBal0097096==T:Avic\GFP<up>EGFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002877==pAGW</fbsym> and <fbsym>FBmc0002878==pAWG</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002909	CCL 1 constr	SGTP 1 composite	SYM 1 pAWH	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 668
ID|FBmc0002909
SYM|pAWH
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAWH
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBgn0015306==T:Ivir\HA1</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002909==pAWH</fbsym> and <fbsym>FBmc0002910==pAHW</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002918	CCL 1 constr	SGTP 1 composite	SYM 1 pAWM	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 668
ID|FBmc0002918
SYM|pAWM
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAWM
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBgn0015310==T:Hsap\MYC</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002917==pAMW</fbsym> and <fbsym>FBmc0002918==pAWM</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002902	CCL 1 constr	SGTP 1 composite	SYM 1 pAWR	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 681
ID|FBmc0002902
SYM|pAWR
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAWR
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | epitope tag | <fbsym>FBal0155770==T:Disc\RFP<up>mRFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002901==pARW</fbsym> and <fbsym>FBmc0002902==pAWR</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002894	CCL 1 constr	SGTP 1 composite	SYM 1 pAWV	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 686
ID|FBmc0002894
SYM|pAWV
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pAWV
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | general promoter | Act5C
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
|cloning vector | epitope tag | <fbsym>FBal0155771==T:Avic\GFP<up>YFP.Venus</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002893==pAVW</fbsym> and <fbsym>FBmc0002894==pAWV</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001479	CCL 1 constr	SGTP 1 composite	SYM 1 pBAC108L	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 879
ID|FBmc0001479
SYM|pBAC108L
TRL|6941
DT|13 May 1999
|4 Feb 1999
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
ENL|NotI (inclusive)
ENR|NotI (exclusive)
CS|HindIII BamHI
TCL|plasmid | BAC
TFT|bacterial selection | camR
TPU|cloning vector | BAC
CC|construct
|Reference: Shizuya, et al., 1992, Proc. Natl. Acad. Sci. USA 89: 8794-8797.
| BAC = bacterial artificial chromosome Unique cleavage sites: cosN, loxP.
PRG|in vitro progenitor <fbsym>FBmc0001471==pMBO131</fbsym>
|in vitro progenitor: P1 bacteriophage sequences (loxP site)
|in vitro progenitor: lambda bacteriophage sequences (cosN site)
|in vitro progenitor: synthetic oligonucleotide (containing restriction sites, T7/SP6 promoters)
DEC|in vitro descendant <fbsym>FBmc0001480==pBACe3.6</fbsym>
|in vitro descendant <fbsym>FBmc0001481==pBeloBAC11</fbsym>
}

REF
{
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002758	CCL 1 constr	SGTP 1 composite	SYM 1 pBac&Dgr;Sac	DBA 1 -	REF 1 2	DT 1 1 May 2001	RESZ 909
ID|FBmc0002758
SYM|pBac&Dgr;Sac
DT|1 May 2001
|24 Apr 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0024718==Tni\piggyBac\T</fbsym>

REFDSR
{
RDID|FBrf0123037
|Handler and Harrell
|1999
SYN|pBacDeltaSac
ASAL|<fbsym>FBal0155753==Tni\piggyBac\T<up>&Dgr;Sac</up></fbsym>
TCL|plasmid
TPU|transposase source | source immobile
|transposase source | <fbsym>FBgn0024718==Tni\piggyBac\T</fbsym> | active in germ-line
CC|construct
|Low efficiency in D. melanogaster; <fbsym>FBmc0002753==phsp-pBac</fbsym> approx. 8-fold more efficient.
}

REFDSR
{
RDID|FBrf0132292
|Horn et al.
|2000
TPU|transposase source | <fbsym>FBgn0024718==Tni\piggyBac\T</fbsym> | source immobile
CC|construct
|Tested in <fbsym>D. melanogaster</fbsym>; <fbsym>FBmc0002753==phsp-pBac</fbsym> found to be more effective than
| <fbsym>pBac&Dgr;Sac</fbsym>.
}

REF
{
REFM|FBrf0123037
|Handler and Harrell
|1999
REFM|FBrf0132292
|Horn et al.
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001480	CCL 1 constr	SGTP 1 composite	SYM 1 pBACe3.6	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 889
ID|FBmc0001480
SYM|pBACe3.6
TRL|11490
DT|13 May 1999
|4 Feb 1999
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
ENL|BamHI (5bp)
ENR|BamHI (1bp)
CS|BamHI HindIII
TCL|plasmid | BAC
TFT|bacterial selection | camR
|selection for inserts | sacBII<up>-</up>
TPU|cloning vector | BAC
CC|construct
|Reference: Web sites <url>http://bacpac.med.buffalo.edu/drosophila_bac.htm</url>
| <url>http://bacpac.med.buffalo.edu/vector.shtmlBAC</url> = bacterial artificial
| chromosome Unique cleavage sites: cosN, loxP, loxP511, PI-SceI, Tn7att. BAC
| vector used by the Berkeley Drosophila Genome Project.
|report
|NOTE: length of vector only (without the 2.683kb stuffer fragment) is 8.807kb.
PRG|in vitro progenitor <fbsym>FBtp0001097==pSacBII</fbsym>
|in vitro progenitor <fbsym>FBmc0001479==pBAC108L</fbsym>
|in vitro progenitor: pUC19
}

REF
{
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001481	CCL 1 constr	SGTP 1 composite	SYM 1 pBeloBAC11	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 753
ID|FBmc0001481
SYM|pBeloBAC11
DT|13 May 1999
|4 Feb 1999
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
ENL|NotI (inclusive)
ENR|NotI (exclusive)
CS|BamHI HindIII
TCL|plasmid | BAC
TFT|bacterial selection | camR
|selection for inserts | lacZ<up>-</up>
TPU|cloning vector | BAC
CC|construct
|Reference: Web site
| <url>http://www.tree.caltech.edu/protocols/pictures/pbelobac.gifBAC</url> = bacterial
| artificial chromosome Unique cleavage sites: cosN, loxP. BAC vector used by
| the European Drosophila Genome Project.
PRG|in vitro progenitor <fbsym>FBmc0001479==pBAC108L</fbsym>
|in vitro progenitor: pBluescribe (Stratagene), modified in vitro to replace T3 promoter with SP6 promoter
}

REF
{
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000726	CCL 1 constr	SGTP 1 composite	SYM 1 pBLCAT2	DBA 1 +	REF 1 1	DT 1 8 Apr 1997	RESZ 786
ID|FBmc0000726
SYM|pBLCAT2
TRL|4496
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
DBA|NA:X64410

REFDSR
{
RDID|FBrf0053349
|Thisse et al.
|1991
SYN|pBLCAT2
TCH|reporter cassette (minimal promoter) | Ecol\CAT
TCL|plasmid
TFT|bacterial selection | ampR
|chimeric gene | Hsim\TK::Ecol\CAT::Simian virus 40
TPU|cloning vector | reporter
|cloning vector | cell culture assay
CC|construct
|CAT vector for transient transfection into eukaryotic cells.  Contains TK
| promoter for analysis of cis-acting elements in front of a heterologous
| promoter. Construction described in Luckow and Schutz, 1987, NAR 15:5490.
DEC|in vitro descendant <fbsym>FBmc0000730==ptwi-CAT</fbsym>
|in vitro descendant <fbsym>FBmc0000732==ptwi(a)-CAT</fbsym>
}

REF
{
REFM|FBrf0053349
|Thisse et al.
|1991
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000727	CCL 1 constr	SGTP 1 composite	SYM 1 pBLCAT3	DBA 1 +	REF 1 4	DT 1 8 Apr 1997	RESZ 1401
ID|FBmc0000727
SYM|pBLCAT3
TRL|4344
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
DBA|NA:X64409
SEG|1 - <fbsym>FBms0000905==pUC18-mcs(H3,R1)</fbsym>
|2 + <fbsym>FBms0003488==blcat.mcs</fbsym>
|3 + <fbsym>FBms0000677==CAT::SV40_1.6(-P)</fbsym>
|4 + <fbsym>FBms0003489==001196</fbsym>
ASGN|<fbsym>FBgn0014442==Ecol\CAT</fbsym>
MMP|FBmc0000727
RSQ|FBmc0000727

REFDSR
{
RDID|FBrf0048083
|Thummel et al.
|1988
ASAL|<fbsym>FBal0040258==Ecol\CAT<up>C4</up></fbsym>
}

REFDSR
{
RDID|FBrf0048799
|Bunch et al.
|1988
TFT|high-copy-number plasmid
|bacterial selection | ampR
}

REFDSR
{
RDID|FBrf0053349
|Thisse et al.
|1991
ENL|EcoRI
ENR|EcoRI (inclusive)
CS|In front of <fbsym>CAT</fbsym> reporter gene: SstI SstII NotI XbaI SpeI PstI EcoRI EcoRV HindIII ClaI XhoI KpnI
SYN|pBLCAT3
TCH|reporter cassette (+ATG) | <fbsym>FBgn0014442==Ecol\CAT</fbsym>
TCL|plasmid
TFT|bacterial selection | ampR
|chimeric gene | Ecol\CAT::Simian virus 40
TPU|cloning vector | reporter
|cloning vector | cell culture assay
CC|construct
|Promoterless <fbsym>CAT</fbsym> vector for transient transfection experiments with
| eukaryotic cells. Allows the analysis of foreign promoters and enhancers.
| Construction described in Luckow and Schutz, 1987, NAR 15:5490.
}

REF
{
REFM|FBrf0048083
|Thummel et al.
|1988
REFM|FBrf0048799
|Bunch et al.
|1988
REFM|FBrf0053349
|Thisse et al.
|1991
REFM|FBrf0172263
|Luckow
|1992.2.6
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001327	CCL 1 constr	SGTP 1 composite	SYM 1 pC70lacZ99	DBA 1 -	REF 1 1	DT 1 30 Dec 1997	RESZ 613
ID|FBmc0001327
SYM|pC70lacZ99
DT|30 Dec 1997
|29 Dec 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0014447==Ecol\lacZ</fbsym>

REFDSR
{
RDID|FBrf0068515
|Gunaratne et al.
|1994
ASAL|<fbsym>FBal0047430==Ecol\lacZ<up>Hsp70Bb.-43</up></fbsym>
TCL|plasmid
TFT|reporter cassette (minimal promoter) | <fbsym>FBal0047430==Ecol\lacZ[Hsp70Bb.-43]</fbsym>
TPU|cloning vector | shuttle vector
PRG|in vitro progenitor <fbsym>FBmc0000257==SP1.1-43</fbsym>
|in vitro progenitor: pBluescript (Stratagene)
DEC|in vitro descendant <fbsym>FBmc0001326==pP{W5-hsp70-lacZ}</fbsym>
}

REF
{
REFM|FBrf0068515
|Gunaratne et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001440	CCL 1 constr	SGTP 1 composite	SYM 1 pcophyg	DBA 1 -	REF 1 2	DT 1 13 May 1999	RESZ 695
ID|FBmc0001440
SYM|pcophyg
TRL|6300
DT|13 May 1999
|3 Aug 1998
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0049300
|Scharloo
|1989
CS|BamHI NotI XhoI
SYN|pcophyg
TCL|plasmid
TFT|bacterial selection | ampR
|selection in cell culture | hygR
|selection in cell culture | hygromycin
TPU|cloning vector | cell culture assay
CC|construct
|Allows stable transformation of Drosophila S2 cells, using hygromycin selection.
PRG|in vitro progenitor <fbsym>FBmc0002789==pcopneo</fbsym>
}

REFDSR
{
RDID|FBrf0102541
|Munroe et al.
|1998.5.12
CS|BamHI NotI XhoI
DEC|in vitro descendant <fbsym>FBmc0001439==pMt-Hy</fbsym>
}

REF
{
REFM|FBrf0049300
|Scharloo
|1989
REFM|FBrf0102541
|Munroe et al.
|1998.5.12
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002789	CCL 1 constr	SGTP 1 composite	SYM 1 pcopneo	DBA 1 -	REF 1 2	DT 1 26 Mar 2002	RESZ 1119
ID|FBmc0002789
SYM|pcopneo
TRL|~6306
DT|26 Mar 2002
|21 Mar 1997
CCL|constr
SGTP|composite
ID2|FBtp0001067
SEG|1 + <fbsym>FBms0003589==cDm5002_0.4</fbsym>
|2 + <fbsym>FBms0003585==copia::neoR</fbsym>
|3 + <fbsym>FBms0003693==BamHI.2</fbsym>
|4 + <fbsym>FBms0003586==pAT153(B,R1)</fbsym>
ASGN|<fbsym>FBgn0014449==Tn\neoR</fbsym>

REFDSR
{
RDID|FBrf0043228
|Rio and Rubin
|1985
CS|BamHI
ASAL|<fbsym>FBal0042320==Tn\neoR<up>LTR</up></fbsym>
TCL|plasmid
TFT|bacterial selection | ampR
|selection in cell culture | <fbsym>FBal0042320==Tn\neoR[LTR]</fbsym>
TPU|cloning vector | cell culture assay
CC|construct
|Cloning vector designed for stable transformation of Drosophila S2 cells.
PRG|in vitro progenitor: pAT153 (sequence entry L08853 | 310759 | 04 Jun 1993)
|in vitro progenitor: pSV2neo (sequence entry U02434 | 413800 | 30 May 1996)
|in vitro progenitor: <fbsym>FBgn0000349==copia</fbsym> LTR sequences from cDm5002 (FBrf0034224)
}

REFDSR
{
RDID|FBrf0049300
|Scharloo
|1989
DEC|in vitro descendant <fbsym>FBmc0001440==pcophyg</fbsym>
}

REF
{
REFM|FBrf0043228
|Rio and Rubin
|1985
REFM|FBrf0049300
|Scharloo
|1989
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001189	CCL 1 constr	SGTP 1 composite	SYM 1 p&Dgr;IVS1	DBA 1 -	REF 1 1	DT 1 30 Dec 1997	RESZ 1399
ID|FBmc0001189
SYM|p&Dgr;IVS1
DT|30 Dec 1997
|24 Jun 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0001189==p&Dgr;IVS1</fbsym>
|<fbsym>FBmc0001188==p&Dgr;IVS1,2</fbsym>
|<fbsym>FBmc0001190==p&Dgr;IVS2</fbsym>
|<fbsym>FBmc0001191==pNS&Dgr;1</fbsym>
|<fbsym>FBmc0001192==pNS&Dgr;2</fbsym>
|<fbsym>FBmc0001193==pNS&Dgr;3</fbsym>
|<fbsym>FBmc0001194==pNS&Dgr;4</fbsym>
|<fbsym>FBmc0001195==pNS&Dgr;5</fbsym>
|<fbsym>FBmc0001196==pNS&Dgr;6</fbsym>
|<fbsym>FBmc0001197==pNS&Dgr;7</fbsym>
|<fbsym>FBmc0001198==pNS&Dgr;8</fbsym>
|<fbsym>FBmc0001199==pNS1186</fbsym>
|<fbsym>FBmc0001200==pWX4009</fbsym>
|<fbsym>FBmc0001201==pWX4014</fbsym>
|<fbsym>FBmc0001202==pWX4015</fbsym>
|<fbsym>FBmc0001203==pWX4016</fbsym>
|<fbsym>FBmc0001204==pWX4017</fbsym>
|<fbsym>FBmc0001205==pWX4063</fbsym>
|<fbsym>FBmc0001206==pWX4064</fbsym>
ASGN|<fbsym>FBgn0000055==Adh</fbsym>

REFDSR
{
RDID|FBrf0049519
|Shen et al.
|1989
ASAL|<fbsym>FBal0028766==Adh<up>&Dgr;IVS1</up></fbsym>
TCL|plasmid
TPU|characterization | <fbsym>FBgn0000055==Adh</fbsym>
|transient assay | larva | <fbsym>FBgn0000055==Adh</fbsym>
CC|construct
|One of a  series of constructs characterizing <fbsym>FBgn0000055==Adh</fbsym> 5' and intronic
| regulatory elements using a transient assay system; see "Associated
| alleles" for further details.
PRG|in vitro progenitor <fbsym>FBmc0000907==pNS1191</fbsym>
}

REF
{
REFM|FBrf0049519
|Shen et al.
|1989
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001188	CCL 1 constr	SGTP 1 composite	SYM 1 p&Dgr;IVS1,2	DBA 1 -	REF 1 1	DT 1 30 Dec 1997	RESZ 1403
ID|FBmc0001188
SYM|p&Dgr;IVS1,2
DT|30 Dec 1997
|24 Jun 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0001189==p&Dgr;IVS1</fbsym>
|<fbsym>FBmc0001188==p&Dgr;IVS1,2</fbsym>
|<fbsym>FBmc0001190==p&Dgr;IVS2</fbsym>
|<fbsym>FBmc0001191==pNS&Dgr;1</fbsym>
|<fbsym>FBmc0001192==pNS&Dgr;2</fbsym>
|<fbsym>FBmc0001193==pNS&Dgr;3</fbsym>
|<fbsym>FBmc0001194==pNS&Dgr;4</fbsym>
|<fbsym>FBmc0001195==pNS&Dgr;5</fbsym>
|<fbsym>FBmc0001196==pNS&Dgr;6</fbsym>
|<fbsym>FBmc0001197==pNS&Dgr;7</fbsym>
|<fbsym>FBmc0001198==pNS&Dgr;8</fbsym>
|<fbsym>FBmc0001199==pNS1186</fbsym>
|<fbsym>FBmc0001200==pWX4009</fbsym>
|<fbsym>FBmc0001201==pWX4014</fbsym>
|<fbsym>FBmc0001202==pWX4015</fbsym>
|<fbsym>FBmc0001203==pWX4016</fbsym>
|<fbsym>FBmc0001204==pWX4017</fbsym>
|<fbsym>FBmc0001205==pWX4063</fbsym>
|<fbsym>FBmc0001206==pWX4064</fbsym>
ASGN|<fbsym>FBgn0000055==Adh</fbsym>

REFDSR
{
RDID|FBrf0049519
|Shen et al.
|1989
ASAL|<fbsym>FBal0028765==Adh<up>&Dgr;IVS1,2</up></fbsym>
TCL|plasmid
TPU|characterization | <fbsym>FBgn0000055==Adh</fbsym>
|transient assay | larva | <fbsym>FBgn0000055==Adh</fbsym>
CC|construct
|One of a  series of constructs characterizing <fbsym>FBgn0000055==Adh</fbsym> 5' and intronic
| regulatory elements using a transient assay system; see "Associated
| alleles" for further details.
PRG|in vitro progenitor <fbsym>FBmc0000907==pNS1191</fbsym>
}

REF
{
REFM|FBrf0049519
|Shen et al.
|1989
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001190	CCL 1 constr	SGTP 1 composite	SYM 1 p&Dgr;IVS2	DBA 1 -	REF 1 1	DT 1 30 Dec 1997	RESZ 1399
ID|FBmc0001190
SYM|p&Dgr;IVS2
DT|30 Dec 1997
|24 Jun 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0001189==p&Dgr;IVS1</fbsym>
|<fbsym>FBmc0001188==p&Dgr;IVS1,2</fbsym>
|<fbsym>FBmc0001190==p&Dgr;IVS2</fbsym>
|<fbsym>FBmc0001191==pNS&Dgr;1</fbsym>
|<fbsym>FBmc0001192==pNS&Dgr;2</fbsym>
|<fbsym>FBmc0001193==pNS&Dgr;3</fbsym>
|<fbsym>FBmc0001194==pNS&Dgr;4</fbsym>
|<fbsym>FBmc0001195==pNS&Dgr;5</fbsym>
|<fbsym>FBmc0001196==pNS&Dgr;6</fbsym>
|<fbsym>FBmc0001197==pNS&Dgr;7</fbsym>
|<fbsym>FBmc0001198==pNS&Dgr;8</fbsym>
|<fbsym>FBmc0001199==pNS1186</fbsym>
|<fbsym>FBmc0001200==pWX4009</fbsym>
|<fbsym>FBmc0001201==pWX4014</fbsym>
|<fbsym>FBmc0001202==pWX4015</fbsym>
|<fbsym>FBmc0001203==pWX4016</fbsym>
|<fbsym>FBmc0001204==pWX4017</fbsym>
|<fbsym>FBmc0001205==pWX4063</fbsym>
|<fbsym>FBmc0001206==pWX4064</fbsym>
ASGN|<fbsym>FBgn0000055==Adh</fbsym>

REFDSR
{
RDID|FBrf0049519
|Shen et al.
|1989
ASAL|<fbsym>FBal0028767==Adh<up>&Dgr;IVS2</up></fbsym>
TCL|plasmid
TPU|characterization | <fbsym>FBgn0000055==Adh</fbsym>
|transient assay | larva | <fbsym>FBgn0000055==Adh</fbsym>
CC|construct
|One of a  series of constructs characterizing <fbsym>FBgn0000055==Adh</fbsym> 5' and intronic
| regulatory elements using a transient assay system; see "Associated
| alleles" for further details.
PRG|in vitro progenitor <fbsym>FBmc0000907==pNS1191</fbsym>
}

REF
{
REFM|FBrf0049519
|Shen et al.
|1989
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000815	CCL 1 constr	SGTP 1 composite	SYM 1 pE2-CAT	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 936
ID|FBmc0000815
SYM|pE2-CAT
SYN|pD-33A3
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASCO|<fbsym>FBmc0000073==pAc-&Dgr;zf</fbsym>
|<fbsym>FBmc0000072==pAc-esg</fbsym>
|<fbsym>FBmc0000815==pE2-CAT</fbsym>
|<fbsym>FBmc0000070==phs-sc</fbsym>
|<fbsym>FBmc0000071==phsp-da</fbsym>

REFDSR
{
RDID|FBrf0076506
|Fuse et al.
|1994
SYN|pD-33A3
TCL|plasmid
TFT|reporter gene | Ecol\CAT
|chimeric gene | E2_box::Adh::Ecol\CAT
TPU|characterization | E2 box
|cell culture assay | S2 cells
|characterization | bHLH-protein binding
CC|construct
|Used in conjunction other members of series to characterize bHLH-protein
| binding to E2 box sequences, assayed by subsequent transcription of CAT
| reporter gene.
|report
|Chimeric reporter gene: three tandom E2-box sequences, <fbsym>FBgn0000055==Adh</fbsym> promoter, <fbsym>CAT</fbsym>
| coding region.
PRG|in vitro progenitor: pD-33 (FBrf0048776)
}

REF
{
REFM|FBrf0076506
|Fuse et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002868	CCL 1 constr	SGTP 1 composite	SYM 1 pEP3005	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 432
ID|FBmc0002868
SYM|pEP3005
SYN|EP3005
|PiggyBac ubiquitin-transposase P replacement vector EP3005
TRL|11038
DT|25 Aug 2004
|23 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0171719
|Griswold
|2002.12.13
SYN|EP3005
TCL|plasmid
TPU|transposase source | Tni\piggyBac\T | source immobile
CC|construct
|Does not contain <fbsym>FBgn0024278==Tni\piggyBac</fbsym> terminal repeats.
}

REF
{
REFM|FBrf0171719
|Griswold
|2002.12.13
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000380	CCL 1 constr	SGTP 1 composite	SYM 1 pGaTN	DBA 1 -	REF 1 9	DT 1 10 Jul 1998	RESZ 2217
ID|FBmc0000380
SYM|pGaTN
SYN|pGATN
TRL|~6116
DT|10 Jul 1998
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 - <fbsym>FBms0000790==pBLP-mcs(K,Sc1)</fbsym>
|2 + <fbsym>FBms0001130==001159</fbsym>
|3 + <fbsym>FBms0003206==Hsp70Bb<up>+</up>0.029</fbsym>
|4 + <fbsym>FBms0003208==001264</fbsym>
|5 + <fbsym>FBms0003543==GAL4::Hsp70Bb</fbsym>
|6 + <fbsym>FBms0000917==001157</fbsym>
ASCO|<fbsym>FBms0003205==pGaTB</fbsym>
|<fbsym>FBmc0000380==pGaTN</fbsym>
MMP|FBmc0000380
RSQ|FBmc0000380

REFDSR
{
RDID|FBrf0049802
|Wilson et al.
|1989
TFT|bacterial selection | ampR
}

REFDSR
{
RDID|FBrf0064375
|Brand and Perrimon
|1993
ENL|SacI
ENR|SacI (inclusive)
CS|NotI
SYN|pGATN
|pGaTN
TCL|plasmid
TFT|binary component | GAL4
|chimeric gene | <fbsym>FBms0003543==GAL4::Hsp70Bb</fbsym>
|reporter cassette (+ATG) | <fbsym>FBgn0014445==Scer\GAL4</fbsym>
TPU|cloning vector | reporter
|cloning vector | binary system
|cloning vector | shuttle vector
CC|construct
|Contains convenient cloning site in front of GAL4-Hsp70 chimeric gene.
|report
|Source and extent of Hsp70 sequences not specified.
PRG|in vitro progenitor <fbsym>FBmc0000293==pHSREM1</fbsym>
|in vitro progenitor: pLKC15 (from L Keegan; contains <fbsym>GAL4</fbsym> sequences)
DEC|in vitro descendant <fbsym>FBmc0000381==pP{GawB}</fbsym>
}

REFDSR
{
RDID|FBrf0076490
|Luo et al.
|1994
DEC|in vitro descendant <fbsym>FBmc0000803==pP{GAL4-elav.L}</fbsym>
}

REFDSR
{
RDID|FBrf0080000
|Dunin-Borkowski and Brown
|1995
DEC|in vitro descendant <fbsym>FBmc0000406==pP{UAS-CD2}</fbsym>
}

REFDSR
{
RDID|FBrf0080027
|Frasch
|1995
DEC|in vitro descendant <fbsym>FBmc0001018==pP{GAL4-Kr.zen.8}</fbsym>
}

REFDSR
{
RDID|FBrf0087706
|Shiga et al.
|1996
DEC|in vitro descendant <fbsym>FBmc0001176==pP{GAL4-btl.S}</fbsym>
}

REFDSR
{
RDID|FBrf0107846
|Lee and Luo
|1999
DEC|transposon descendant <fbsym>FBtp0002651==P{tubP-GAL4}</fbsym>
}

REF
{
REFM|FBrf0049802
|Wilson et al.
|1989
REFM|FBrf0064375
|Brand and Perrimon
|1993
REFM|FBrf0076490
|Luo et al.
|1994
REFM|FBrf0076602
|Brand et al.
|1994
REFM|FBrf0080000
|Dunin-Borkowski and Brown
|1995
REFM|FBrf0080027
|Frasch
|1995
REFM|FBrf0087706
|Shiga et al.
|1996
REFM|FBrf0105495
|FlyBase
|1992-
REFM|FBrf0107846
|Lee and Luo
|1999
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001462	CCL 1 constr	SGTP 1 composite	SYM 1 pHBL1	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 531
ID|FBmc0001462
SYM|pHBL1
SYN|HBL1
DT|13 May 1999
|30 Sep 1998
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0014191==hobo\T</fbsym>

REFDSR
{
RDID|FBrf0069031
|Calvi
|1993
SYN|HBL1
ASAL|<fbsym>FBal0034793==hobo\T<up>+t2854</up></fbsym>
TCL|plasmid
TPU|transposase source | <fbsym>FBgn0014191==hobo\T</fbsym> | source immobile
|transposase source | <fbsym>FBgn0014191==hobo\T</fbsym> | active in germ-line
CC|construct
|Plasmid-borne source of <fbsym>FBtp0011431==H-element</fbsym> transposase.
}

REF
{
REFM|FBrf0069031
|Calvi
|1993
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002883	CCL 1 constr	SGTP 1 composite	SYM 1 pHCW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 683
ID|FBmc0002883
SYM|pHCW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHCW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBal0141352==T:Avic\GFP<up>ECFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002883==pHCW</fbsym> and <fbsym>FBmc0002884==pHWC</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002812	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-ECFP,&agr;tub-piggyBacK10}	DBA 1 -	REF 1 1	DT 1 24 Aug 2003	RESZ 700
ID|FBmc0002812
SYM|pHerm{3xP3-ECFP,&agr;tub-piggyBacK10}
SYN|pHer{3xP3-ECFP,alphatub-piggyBacK10}
DT|24 Aug 2003
|22 Aug 2003
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
SYN|pHer{3xP3-ECFP,alphatub-piggyBacK10}
TCL|plasmid
TPU|transposase source | Tni\piggyBac\T
CC|construct
|An AscI fragment from pSLfa_&agr;tub-piggyBacK10_fa has been cloned into
| <fbsym>FBmc0002771==pHerm{3xP3-ECFPaf}</fbsym>.
PRG|in vitro progenitor <fbsym>FBmc0002771==pHerm{3xP3-ECFPaf}</fbsym>
|in vitro progenitor: pSLfa_&agr;tub-piggyBacK10_fa (Exelixis)
DEC|transposon descendant <fbsym>FBtp0017474==Herm{3xP3-ECFP,&agr;tub-piggyBacK10}</fbsym>
}

REF
{
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002810	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-ECFP,hsp70-piggyBac}	DBA 1 -	REF 1 1	DT 1 24 Aug 2003	RESZ 640
ID|FBmc0002810
SYM|pHerm{3xP3-ECFP,hsp70-piggyBac}
SYN|pHer{3xP3-ECFP,hsp70-piggyBac}
DT|24 Aug 2003
|22 Aug 2003
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
SYN|pHer{3xP3-ECFP,hsp70-piggyBac}
TCL|plasmid
TPU|transposase source | Tni\piggyBac\T
CC|construct
|An AscI fragment from <fbsym>FBmc0002809==pSLfa_hsp70-piggyBac_fa</fbsym> has been cloned into the
| AscI site of <fbsym>FBmc0002771==pHerm{3xP3-ECFPaf}</fbsym>.
PRG|in vitro progenitor <fbsym>FBmc0002771==pHerm{3xP3-ECFPaf}</fbsym>
|in vitro progenitor <fbsym>FBmc0002809==pSLfa_hsp70-piggyBac_fa</fbsym>
}

REF
{
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002824	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-ECFP,UASp-EYFP-K10}	DBA 1 -	REF 1 1	DT 1 24 Aug 2003	RESZ 463
ID|FBmc0002824
SYM|pHerm{3xP3-ECFP,UASp-EYFP-K10}
SYN|pHer{3xP3-ECFP,UASp-EYFP-K10}
DT|24 Aug 2003
|22 Aug 2003
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
SYN|pHer{3xP3-ECFP,UASp-EYFP-K10}
TCL|plasmid
TPU|binary system (targeted) | Avic\GFP
PRG|in vitro progenitor: pSLfa_UASp-eyfp-K10_fa (FBrf0158985)
DEC|transposon descendant <fbsym>FBtp0017482==Herm{3xP3-ECFP,UASp-EYFP-K10}</fbsym>
}

REF
{
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002829	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-ECFP,UASp-lacZ-K10}	DBA 1 -	REF 1 1	DT 1 24 Aug 2003	RESZ 464
ID|FBmc0002829
SYM|pHerm{3xP3-ECFP,UASp-lacZ-K10}
SYN|pHer{3xP3-ECFP,UASp-lacZ-K10}
DT|24 Aug 2003
|22 Aug 2003
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
SYN|pHer{3xP3-ECFP,UASp-lacZ-K10}
TCL|plasmid
TPU|binary system (targeted) | Ecol\lacZ
PRG|in vitro progenitor: pSLfa_UASp-lacZ-K10_fa (FBrf0158985)
DEC|transposon descendant <fbsym>FBtp0017484==Herm{3xP3-ECFP,UASp-lacZ-K10}</fbsym>
}

REF
{
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002771	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-ECFPaf}	DBA 1 -	REF 1 2	DT 1 4 Jun 2001	RESZ 1316
ID|FBmc0002771
SYM|pHerm{3xP3-ECFPaf}
SYN|pHer{3xP3-ECFPaf}
DT|4 Jun 2001
|29 May 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0014446==Avic\GFP</fbsym>

REFDSR
{
RDID|FBrf0134553
|Horn and Wimmer
|2000
CS|AscI FseI
SYN|pHer{3xP3-ECFPaf}
ASAL|<fbsym>FBal0122323==Avic\GFP<up>ECFP.3xP3</up></fbsym>
TCL|plasmid
TFT|bacterial selection | ampR
|selectable marker | <fbsym>FBal0122323==Avic\GFP[ECFP.3xP3]</fbsym>
TPU|cloning vector | transposon | Mdoa\Hermes
CC|construct
|One of a series of transposon cloning vectors using the transposon
| <fbsym>FBgn0016680==Mdoa\Hermes</fbsym> as the transfection agent and spectral variants of the
| selectable marker <fbsym>FBgn0014446==Avic\GFP</fbsym>. Designed for use with the shuttle vector
| <fbsym>FBmc0002761==pSLfa1180fa</fbsym>.
PRG|in vitro progenitor <fbsym>FBmc0002764==pSL-3xP3-ECFPaf</fbsym>
|in vitro progenitor: pHermes<up>EGFP</up> (Pinkerton et al., 2000, Insect Mol Biol 9:1-10)
DEC|transposon descendant <fbsym>FBtp0014065==Herm{3xP3-ECFPaf}</fbsym>
}

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
DEC|in vitro descendant <fbsym>FBmc0002810==pHerm{3xP3-ECFP,hsp70-piggyBac}</fbsym>
|in vitro descendant <fbsym>FBmc0002812==pHerm{3xP3-ECFP,&agr;tub-piggyBacK10}</fbsym>
}

REF
{
REFM|FBrf0134553
|Horn and Wimmer
|2000
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002782	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-EGFPaf-OriKan}	DBA 1 -	REF 1 1	DT 1 4 Jun 2001	RESZ 632
ID|FBmc0002782
SYM|pHerm{3xP3-EGFPaf-OriKan}
SYN|pHer{3xP3-EGFPaf-OriKan}
DT|4 Jun 2001
|29 May 2001
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0134553
|Horn and Wimmer
|2000
SYN|pHer{3xP3-EGFPaf-OriKan}
TCL|plasmid
CC|construct
|Donor plasmid for plasmid-based embryonic transposition assay.  Allows test
| of efficacy of transposition in embryos of various targeted species.
|  Target plasmid carries second antibiotic resistance marker; also requires
| a helper plasmid to provide functional transposase.
PRG|in vitro progenitor <fbsym>FBmc0002772==pHerm{3xP3-EGFPaf}</fbsym>
}

REF
{
REFM|FBrf0134553
|Horn and Wimmer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002772	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-EGFPaf}	DBA 1 -	REF 1 1	DT 1 4 Jun 2001	RESZ 1189
ID|FBmc0002772
SYM|pHerm{3xP3-EGFPaf}
SYN|pHer{3xP3-EGFPaf}
DT|4 Jun 2001
|29 May 2001
CCL|constr
SGTP|composite
ASTR|<fbsym>FBtr0006817==Avic\GFP<up>E.3xP3</up>RA</fbsym>
ASGN|<fbsym>FBgn0014446==Avic\GFP</fbsym>

REFDSR
{
RDID|FBrf0134553
|Horn and Wimmer
|2000
CS|AscI FseI
SYN|pHer{3xP3-EGFPaf}
ASAL|<fbsym>FBal0103092==Avic\GFP<up>E.3xP3</up></fbsym>
TCL|plasmid
TFT|bacterial selection | ampR
|selectable marker | <fbsym>FBal0103092==Avic\GFP[E.3xP3]</fbsym>
TPU|cloning vector | transposon | Mdoa\Hermes
CC|construct
|One of a series of transposon cloning vectors using the transposon
| <fbsym>FBgn0016680==Mdoa\Hermes</fbsym> as the transfection agent and spectral variants of the
| selectable marker <fbsym>FBgn0014446==Avic\GFP</fbsym>. Designed for use with the shuttle vector
| <fbsym>FBmc0002761==pSLfa1180fa</fbsym>.
PRG|in vitro progenitor <fbsym>FBmc0002762==pSL-3xP3-EGFPaf</fbsym>
|in vitro progenitor: pHermes<up>EGFP</up> (Pinkerton et al., 2000, Insect Mol Biol 9:1-10)
DEC|in vitro descendant <fbsym>FBmc0002782==pHerm{3xP3-EGFPaf-OriKan}</fbsym>
|transposon descendant <fbsym>FBtp0014066==Herm{3xP3-EGFPaf}</fbsym>
}

REF
{
REFM|FBrf0134553
|Horn and Wimmer
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002756	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-EGFP}	DBA 1 -	REF 1 1	DT 1 1 May 2001	RESZ 1124
ID|FBmc0002756
SYM|pHerm{3xP3-EGFP}
SYN|pHer{3xP3-EGFP}
DT|1 May 2001
|24 Apr 2001
CCL|constr
SGTP|composite
ASTR|<fbsym>FBtr0006817==Avic\GFP<up>E.3xP3</up>RA</fbsym>
ASGN|<fbsym>FBgn0014446==Avic\GFP</fbsym>

REFDSR
{
RDID|FBrf0132292
|Horn et al.
|2000
SYN|pHer{3xP3-EGFP}
ASAL|<fbsym>FBal0103092==Avic\GFP<up>E.3xP3</up></fbsym>
TCL|plasmid
TFT|selectable marker | <fbsym>FBal0103092==Avic\GFP[E.3xP3]</fbsym>
TPU|cloning vector | transposon | Mdoa\Hermes
CC|construct
|Non-autonomous; <fbsym>FBgn0016681==Mdoa\Hermes\T</fbsym> disrupted. Tested in <fbsym>D.melanogaster</fbsym>.
|report
|GFP expression driven by multimer of an idealized <fbsym>FBgn0005558==ey</fbsym>
|/<fbsym>FBgn0016101==Mmus\Pax6</fbsym> binding site (P3), resulting in high levels of
| expression in the eye.
PRG|in vitro progenitor: Hsp70 genomic sequences
|in vitro progenitor: <fbsym>FBgn0016101==Mmus\Pax6</fbsym> homodimer binding sites
|in vitro progenitor: pHermes{EGFP} (Pinkerton et al., 2000, Insect Mol. Biol. 9:1--10)
DEC|transposon descendant <fbsym>FBtp0012983==Herm{3xP3-EGFP}</fbsym>
}

REF
{
REFM|FBrf0132292
|Horn et al.
|2000
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002813	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-EYFP,&agr;tub-piggyBacK10}	DBA 1 -	REF 1 1	DT 1 24 Aug 2003	RESZ 609
ID|FBmc0002813
SYM|pHerm{3xP3-EYFP,&agr;tub-piggyBacK10}
SYN|pHer{3xP3-EYFP,alphatub-piggyBacK10}
DT|24 Aug 2003
|22 Aug 2003
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
SYN|pHer{3xP3-EYFP,alphatub-piggyBacK10}
TCL|plasmid
TPU|transposase source | Tni\piggyBac\T
CC|construct
|An AscI fragment from pSLfa_&agr;tub-piggyBacK10_fa has been cloned into
| <fbsym>FBmc0002773==pHerm{3xP3-EYFPaf}</fbsym>.
PRG|in vitro progenitor <fbsym>FBmc0002773==pHerm{3xP3-EYFPaf}</fbsym>
|in vitro progenitor: pSLfa_&agr;tub-piggyBacK10_fa (Exelixis)
}

REF
{
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002816	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-EYFP,p-GAL4&Dgr;-K10}	DBA 1 -	REF 1 1	DT 1 24 Aug 2003	RESZ 594
ID|FBmc0002816
SYM|pHerm{3xP3-EYFP,p-GAL4&Dgr;-K10}
SYN|pHer{3xP3-EYFP,p-GAL4Delta-K10}
DT|24 Aug 2003
|22 Aug 2003
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
SYN|pHer{3xP3-EYFP,p-GAL4Delta-K10}
TCL|plasmid
TPU|enhancer trap | binary system
CC|construct
|An AscI fragment from pSL_p-GAL4&Dgr;-K10_fa has been cloned into the AscI
| site of <fbsym>FBmc0002773==pHerm{3xP3-EYFPaf}</fbsym>.
PRG|in vitro progenitor <fbsym>FBmc0002773==pHerm{3xP3-EYFPaf}</fbsym>
|in vitro progenitor: pSL_p-GAL4&Dgr;-K10_fa (FBrf0158985)
}

REF
{
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002825	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-EYFP,UASp-EYFP-K10}	DBA 1 -	REF 1 1	DT 1 24 Aug 2003	RESZ 855
ID|FBmc0002825
SYM|pHerm{3xP3-EYFP,UASp-EYFP-K10}
SYN|pHer{3xP3-EYFP,UASp-EYFP-K10}
DT|24 Aug 2003
|22 Aug 2003
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
SYN|pHer{3xP3-EYFP,UASp-EYFP-K10}
TCL|plasmid
TPU|binary system (targeted)
CC|construct
|It is possible that the <fbsym>FBgn0015319==Scer\UAS</fbsym> regulatory sequences present downstream
| of the <fbsym>FBal0122322==Avic\GFP<up>EYFP.3xP3</up></fbsym> marker allele in
| <fbsym>FBmc0002825==pHerm{3xP3-EYFP,UASp-EYFP-K10}</fbsym> may affect the expression of
| <fbsym>FBal0122322==Avic\GFP<up>EYFP.3xP3</up></fbsym> once it is transformed into flies
| (as has been shown for the similar construct
| <fbsym>FBmc0002824==pHerm{3xP3-ECFP,UASp-EYFP-K10}</fbsym>).
PRG|in vitro progenitor: pSLfa_UASp-eyfp-K10_fa (FBrf0158985)
}

REF
{
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002830	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-EYFP,UASp-lacZ-K10}	DBA 1 -	REF 1 1	DT 1 24 Aug 2003	RESZ 380
ID|FBmc0002830
SYM|pHerm{3xP3-EYFP,UASp-lacZ-K10}
SYN|pHer{3xP3-EYFP,UASp-lacZ-K10}
DT|24 Aug 2003
|22 Aug 2003
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
SYN|pHer{3xP3-EYFP,UASp-lacZ-K10}
TCL|plasmid
TPU|binary system (targeted) | Ecol\lacZ
PRG|in vitro progenitor: pSLfa_UASp-lacZ-K10_fa (FBrf0158985)
}

REF
{
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002773	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{3xP3-EYFPaf}	DBA 1 -	REF 1 2	DT 1 4 Jun 2001	RESZ 1317
ID|FBmc0002773
SYM|pHerm{3xP3-EYFPaf}
SYN|pHer{3xP3-EYFPaf}
DT|4 Jun 2001
|29 May 2001
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0014446==Avic\GFP</fbsym>

REFDSR
{
RDID|FBrf0134553
|Horn and Wimmer
|2000
CS|AscI FseI
SYN|pHer{3xP3-EYFPaf}
ASAL|<fbsym>FBal0122322==Avic\GFP<up>EYFP.3xP3</up></fbsym>
TCL|plasmid
TFT|bacterial selection | ampR
|selectable marker | <fbsym>FBal0122322==Avic\GFP[EYFP.3xP3]</fbsym>
TPU|cloning vector | transposon | Mdoa\Hermes
CC|construct
|One of a series of transposon cloning vectors using the transposon
| <fbsym>FBgn0016680==Mdoa\Hermes</fbsym> as the transfection agent and spectral variants of the
| selectable marker <fbsym>FBgn0014446==Avic\GFP</fbsym>. Designed for use with the shuttle vector
| <fbsym>FBmc0002761==pSLfa1180fa</fbsym>.
PRG|in vitro progenitor <fbsym>FBmc0002763==pSL-3xP3-EYFPaf</fbsym>
|in vitro progenitor: pHermes<up>EGFP</up> (Pinkerton et al., 2000, Insect Mol Biol 9:1-10)
DEC|transposon descendant <fbsym>FBtp0014067==Herm{3xP3-EYFPaf}</fbsym>
}

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
DEC|in vitro descendant <fbsym>FBmc0002813==pHerm{3xP3-EYFP,&agr;tub-piggyBacK10}</fbsym>
|in vitro descendant <fbsym>FBmc0002816==pHerm{3xP3-EYFP,p-GAL4&Dgr;-K10}</fbsym>
}

REF
{
REFM|FBrf0134553
|Horn and Wimmer
|2000
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002836	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{M{SalW}}	DBA 1 -	REF 1 1	DT 1 3 Mar 2004	RESZ 386
ID|FBmc0002836
SYM|pHerm{M{SalW}}
DT|3 Mar 2004
|3 Mar 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0144896
|Lozovsky et al.
|2002
SYN|pHerm{M{SalW}}
TCL|plasmid
TPU|characterization | Dmau\mariner\T
PRG|in vitro progenitor <fbsym>FBmc0001470==pM{peach}</fbsym>
DEC|transposon descendant <fbsym>FBtp0018161==Herm{M{SalW}}</fbsym>
}

REF
{
REFM|FBrf0144896
|Lozovsky et al.
|2002
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002837	CCL 1 constr	SGTP 1 composite	SYM 1 pHerm{M{SphW}}	DBA 1 -	REF 1 1	DT 1 3 Mar 2004	RESZ 386
ID|FBmc0002837
SYM|pHerm{M{SphW}}
DT|3 Mar 2004
|3 Mar 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0144896
|Lozovsky et al.
|2002
SYN|pHerm{M{SphW}}
TCL|plasmid
TPU|characterization | Dmau\mariner\T
PRG|in vitro progenitor <fbsym>FBmc0001470==pM{peach}</fbsym>
DEC|transposon descendant <fbsym>FBtp0018163==Herm{M{SphW}}</fbsym>
}

REF
{
REFM|FBrf0144896
|Lozovsky et al.
|2002
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002929	CCL 1 constr	SGTP 1 composite	SYM 1 pHFHW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 637
ID|FBmc0002929
SYM|pHFHW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHFHW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBgn0015306==T:Ivir\HA1</fbsym>
|cloning vector | epitope tag | <fbsym>FBgn0015304==T:Zzzz\FLAG</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002933	CCL 1 constr	SGTP 1 composite	SYM 1 pHFMW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 637
ID|FBmc0002933
SYM|pHFMW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHFMW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBgn0015310==T:Hsap\MYC</fbsym>
|cloning vector | epitope tag | <fbsym>FBgn0015304==T:Zzzz\FLAG</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002923	CCL 1 constr	SGTP 1 composite	SYM 1 pHFW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 671
ID|FBmc0002923
SYM|pHFW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHFW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBgn0015304==T:Zzzz\FLAG</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002923==pHFW</fbsym> and <fbsym>FBmc0002924==pHWF</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001075	CCL 1 constr	SGTP 1 composite	SYM 1 pHGCO	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 848
ID|FBmc0001075
SYM|pHGCO
TRL|~5900
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 + <fbsym>FBms0003643==pBR322(B,R1)</fbsym>
|2 + <fbsym>FBms0003299==EcoRI</fbsym>
|3 + <fbsym>FBms0003644==SV40(blip)</fbsym>
|4 + <fbsym>FBms0003637==001200</fbsym>
|5 + <fbsym>FBms0003630==copia::DHFR</fbsym>
|6 + <fbsym>FBms0003236==BamHI</fbsym>
MMP|FBmc0001075
RSQ|FBmc0001075

REFDSR
{
RDID|FBrf0039281
|Bourouis and Jarry
|1983
ENL|BamHI
ENR|BamHI (inclusive)
CS|SalI PvuII
ASAL|DHFR<up>cop</up>
TCL|plasmid
TFT|selection in cell culture | <fbsym>DHFR<up>cop</up></fbsym>
TPU|cloning vector | cell culture assay
CC|construct
|Cloning vector designed for stable transformation of Drosophila Kc cells.
PRG|in vitro progenitor: pHG (O'Hare et al., 1981, Proc. Natl. Acad. Sci. USA, 78:1527-1531)
}

REF
{
REFM|FBrf0039281
|Bourouis and Jarry
|1983
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002875	CCL 1 constr	SGTP 1 composite	SYM 1 pHGW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 683
ID|FBmc0002875
SYM|pHGW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHGW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBal0097096==T:Avic\GFP<up>EGFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002875==pHGW</fbsym> and <fbsym>FBmc0002876==pHWG</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002908	CCL 1 constr	SGTP 1 composite	SYM 1 pHHW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 670
ID|FBmc0002908
SYM|pHHW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHHW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBgn0015306==T:Ivir\HA1</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002907==pHWH</fbsym> and <fbsym>FBmc0002908==pHHW</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002915	CCL 1 constr	SGTP 1 composite	SYM 1 pHMW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 670
ID|FBmc0002915
SYM|pHMW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHMW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBgn0015310==T:Hsap\MYC</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002915==pHMW</fbsym> and <fbsym>FBmc0002916==pHWM</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002899	CCL 1 constr	SGTP 1 composite	SYM 1 pHRW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 683
ID|FBmc0002899
SYM|pHRW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHRW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBal0155770==T:Disc\RFP<up>mRFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002899==pHRW</fbsym> and <fbsym>FBmc0002900==pHWR</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000523	CCL 1 constr	SGTP 1 composite	SYM 1 phs-&pgr;	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 757
ID|FBmc0000523
SYM|phs-&pgr;
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0013311==P\T</fbsym>

REFDSR
{
RDID|FBrf0045018
|Steller and Pirrotta
|1986
ASAL|<fbsym>FBal0038947==P\T<up>hs.PSa</up></fbsym>
TCL|plasmid
TFT|complete rescue | <fbsym>FBal0038947==P\T[hs.PSa]</fbsym>
TPU|transposase source | <fbsym>FBgn0013311==P\T</fbsym> | source immobile
|transposase source | <fbsym>FBgn0013311==P\T</fbsym> | active in germ-line
CC|construct
|P3' end removed by excision of BamHI-EcoRV fragment from
| <fbsym>pP{Icarus&Dgr;Bam-}</fbsym>.
|report
|P-element transposase under control of heat-shock promoter.
PRG|in vitro progenitor <fbsym>FBmc0000522==pP{Icarus&Dgr;Bam-}</fbsym>
}

REF
{
REFM|FBrf0045018
|Steller and Pirrotta
|1986
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000070	CCL 1 constr	SGTP 1 -	SYM 1 phs-sc	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 712
ID|FBmc0000070
SYM|phs-sc
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
ASCO|<fbsym>FBmc0000073==pAc-&Dgr;zf</fbsym>
|<fbsym>FBmc0000072==pAc-esg</fbsym>
|<fbsym>FBmc0000815==pE2-CAT</fbsym>
|<fbsym>FBmc0000070==phs-sc</fbsym>
|<fbsym>FBmc0000071==phsp-da</fbsym>

REFDSR
{
RDID|FBrf0076506
|Fuse et al.
|1994
TPU|characterization | sc
|characterization | E2 box
|cell culture assay | S2 cells
|characterization | bHLH-protein binding
CC|construct
|Used in conjunction with <fbsym>FBmc0000815==pE2-CAT</fbsym> and other members of series to
| characterize bHLH-protein binding to E2 box sequences, assayed by
| subsequent transcription of <fbsym>CAT</fbsym> reporter gene.
}

REF
{
REFM|FBrf0076506
|Fuse et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002618	CCL 1 constr	SGTP 1 composite	SYM 1 pHSHH1.9	DBA 1 -	REF 1 1	DT 1 5 Nov 1999	RESZ 835
ID|FBmc0002618
SYM|pHSHH1.9
DT|5 Nov 1999
|3 Nov 1999
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0016681==Mdoa\Hermes\T</fbsym>

REFDSR
{
RDID|FBrf0086560
|O'Brochta et al.
|1996
SYN|pHSHH1.9
ASAL|<fbsym>FBal0048883==Mdoa\Hermes\T<up>hs.PO</up></fbsym>
TCL|plasmid
TFT|general promoter | <fbsym>FBal0048883==Mdoa\Hermes\T[hs.PO]</fbsym>
|inducible promoter | <fbsym>FBal0048883==Mdoa\Hermes\T[hs.PO]</fbsym>
TPU|heat-shock construct | <fbsym>FBgn0016681==Mdoa\Hermes\T</fbsym>
|transposase source | <fbsym>FBgn0016681==Mdoa\Hermes\T</fbsym> | source immobile
|transposase source | <fbsym>FBgn0016681==Mdoa\Hermes\T</fbsym> | active in germ-line and soma
PRG|in vitro progenitor: Hsp70 genomic sequences
|in vitro progenitor: <fbsym>FBgn0016681==Mdoa\Hermes\T</fbsym> coding sequences
}

REF
{
REFM|FBrf0086560
|O'Brochta et al.
|1996
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000071	CCL 1 constr	SGTP 1 -	SYM 1 phsp-da	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 713
ID|FBmc0000071
SYM|phsp-da
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
ASCO|<fbsym>FBmc0000073==pAc-&Dgr;zf</fbsym>
|<fbsym>FBmc0000072==pAc-esg</fbsym>
|<fbsym>FBmc0000815==pE2-CAT</fbsym>
|<fbsym>FBmc0000070==phs-sc</fbsym>
|<fbsym>FBmc0000071==phsp-da</fbsym>

REFDSR
{
RDID|FBrf0076506
|Fuse et al.
|1994
TPU|characterization | da
|characterization | E2 box
|cell culture assay | S2 cells
|characterization | bHLH-protein binding
CC|construct
|Used in conjunction with <fbsym>FBmc0000815==pE2-CAT</fbsym> and other members of series to
| characterize bHLH-protein binding to E2 box sequences, assayed by
| subsequent transcription of <fbsym>CAT</fbsym> reporter gene.
}

REF
{
REFM|FBrf0076506
|Fuse et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002753	CCL 1 constr	SGTP 1 composite	SYM 1 phsp-pBac	DBA 1 -	REF 1 4	DT 1 1 May 2001	RESZ 972
ID|FBmc0002753
SYM|phsp-pBac
DT|1 May 2001
|24 Apr 2001
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0123037
|Handler and Harrell
|1999
TPU|transposase source | <fbsym>FBgn0024718==Tni\piggyBac\T</fbsym> | source immobile
CC|construct
|Tested in <fbsym>D. melanogaster</fbsym>.
}

REFDSR
{
RDID|FBrf0132292
|Horn et al.
|2000
SYN|phsp-pBac
TCL|plasmid
TPU|transposase source | <fbsym>FBgn0024718==Tni\piggyBac\T</fbsym> | source immobile
|transposase source | <fbsym>FBgn0024718==Tni\piggyBac\T</fbsym> | active in germ-line
CC|construct
|Tested in <fbsym>D. melanogaster</fbsym>; <fbsym>FBmc0002753==phsp-pBac</fbsym> found to be more effective than
| <fbsym>pBac&Dgr;Sac</fbsym>.
}

REFDSR
{
RDID|FBrf0158985
|Horn et al.
|2003
DEC|in vitro descendant <fbsym>FBmc0002809==pSLfa_hsp70-piggyBac_fa</fbsym>
}

REF
{
REFM|FBrf0123037
|Handler and Harrell
|1999
REFM|FBrf0125140
|Bai and Tolias
|2000
REFM|FBrf0132292
|Horn et al.
|2000
REFM|FBrf0158985
|Horn et al.
|2003
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000293	CCL 1 constr	SGTP 1 composite	SYM 1 pHSREM1	DBA 1 -	REF 1 4	DT 1 8 Apr 1997	RESZ 1487
ID|FBmc0000293
SYM|pHSREM1
TRL|~3517
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 - <fbsym>FBms0000790==pBLP-mcs(K,Sc1)</fbsym>
|2 + <fbsym>FBms0000913==001155</fbsym>
|3 + <fbsym>FBms0000914==Hsp70Bb<up>+</up>0.3(X1,P)</fbsym>
|4 + <fbsym>FBms0000915==001156</fbsym>
|5 + <fbsym>FBms0000916==001151</fbsym>
|6 + <fbsym>FBms0000738==Hsp70Bb<up>+</up>0.2(S,Bg2)</fbsym>
|7 + <fbsym>FBms0000917==001157</fbsym>
RSQ|FBmc0000293

REFDSR
{
RDID|FBrf0048840
|Knipple and Marsella-Herrick
|1988
ENL|SacI
ENR|SacI (inclusive)
CS|Between Hsp70b sequences: PstI HindIII EcoRV EcoRI SmaI BamHI ClaI SalI
TCL|plasmid
TPU|shuttle construct
|construction intermediate
CC|construct
|Contains multiple cloning sites flanked by Hsp70 promoter and 3' termination sequences.
|report
|Source and extent of Hsp70 sequences not specified.
PRG|in vitro progenitor: <fbsym>FBgn0013278==Hsp70Bb</fbsym> genomic regions
|in vitro progenitor: pBluescript_KS+ (sequence entry X52331 | 58065 | 10 May 1995)
}

REFDSR
{
RDID|FBrf0049802
|Wilson et al.
|1989
TFT|bacterial selection | ampR
}

REFDSR
{
RDID|FBrf0064375
|Brand and Perrimon
|1993
DEC|in vitro descendant <fbsym>FBmc0000380==pGaTN</fbsym>
}

REFDSR
{
RDID|FBrf0076602
|Brand et al.
|1994
DEC|in vitro descendant <fbsym>FBmc0000404==pP{GAL4-Hsp70.PB}</fbsym>
}

REF
{
REFM|FBrf0048840
|Knipple and Marsella-Herrick
|1988
REFM|FBrf0049802
|Wilson et al.
|1989
REFM|FBrf0064375
|Brand and Perrimon
|1993
REFM|FBrf0076602
|Brand et al.
|1994
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001268	CCL 1 constr	SGTP 1 composite	SYM 1 pHSS6hsMi2	DBA 1 -	REF 1 2	DT 1 10 Aug 1998	RESZ 723
ID|FBmc0001268
SYM|pHSS6hsMi2
TRL|4300
DT|10 Aug 1998
|24 Jun 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0013814==Dhyd\Minos\T</fbsym>

REFDSR
{
RDID|FBrf0084149
|Loukeris et al.
|1995
ASAL|<fbsym>FBal0033602==Dhyd\Minos\T<up>hs.PF</up></fbsym>
TCL|plasmid
TPU|transposase source | <fbsym>FBgn0013814==Dhyd\Minos\T</fbsym> | source immobile
PRG|in vitro progenitor: <fbsym>FBgn0010242==Dhyd\Minos</fbsym> sequences
|in vitro progenitor: <fbsym>FBgn0013278==Hsp70Bb</fbsym> genomic sequences
|in vitro progenitor: pHSS6 (sequence entry M84115 | 146531 | 01-JUN-1994)
DEC|in vitro descendant <fbsym>FBmc0001229==pP{hsMi2}</fbsym>
}

REF
{
REFM|FBrf0084149
|Loukeris et al.
|1995
REFM|FBrf0085479
|Loukeris et al.
|1995
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002891	CCL 1 constr	SGTP 1 composite	SYM 1 pHVW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 688
ID|FBmc0002891
SYM|pHVW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHVW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
|cloning vector | epitope tag | <fbsym>FBal0155771==T:Avic\GFP<up>YFP.Venus</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002891==pHVW</fbsym> and <fbsym>FBmc0002892==pHWV</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002937	CCL 1 constr	SGTP 1 composite	SYM 1 pHW	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 490
ID|FBmc0002937
SYM|pHW
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHW
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002884	CCL 1 constr	SGTP 1 composite	SYM 1 pHWC	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 683
ID|FBmc0002884
SYM|pHWC
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHWC
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBal0141352==T:Avic\GFP<up>ECFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002883==pHCW</fbsym> and <fbsym>FBmc0002884==pHWC</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002924	CCL 1 constr	SGTP 1 composite	SYM 1 pHWF	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 671
ID|FBmc0002924
SYM|pHWF
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHWF
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBgn0015304==T:Zzzz\FLAG</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002923==pHFW</fbsym> and <fbsym>FBmc0002924==pHWF</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002876	CCL 1 constr	SGTP 1 composite	SYM 1 pHWG	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 683
ID|FBmc0002876
SYM|pHWG
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHWG
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBal0097096==T:Avic\GFP<up>EGFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002875==pHGW</fbsym> and <fbsym>FBmc0002876==pHWG</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002907	CCL 1 constr	SGTP 1 composite	SYM 1 pHWH	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 670
ID|FBmc0002907
SYM|pHWH
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHWH
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBgn0015306==T:Ivir\HA1</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002907==pHWH</fbsym> and <fbsym>FBmc0002908==pHHW</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002916	CCL 1 constr	SGTP 1 composite	SYM 1 pHWM	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 670
ID|FBmc0002916
SYM|pHWM
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHWM
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBgn0015310==T:Hsap\MYC</fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002915==pHMW</fbsym> and <fbsym>FBmc0002916==pHWM</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002900	CCL 1 constr	SGTP 1 composite	SYM 1 pHWR	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 683
ID|FBmc0002900
SYM|pHWR
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHWR
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | epitope tag | <fbsym>FBal0155770==T:Disc\RFP<up>mRFP</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002899==pHRW</fbsym> and <fbsym>FBmc0002900==pHWR</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0002892	CCL 1 constr	SGTP 1 composite	SYM 1 pHWV	DBA 1 -	REF 1 1	DT 1 25 Aug 2004	RESZ 688
ID|FBmc0002892
SYM|pHWV
DT|25 Aug 2004
|25 Aug 2004
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0179058
|Murphy
|2003-
SYN|pHWV
TCL|plasmid
TPU|cloning vector | cell culture assay
|cloning vector | inducible promoter | Hsp70
|cloning vector | recombinational | <fbsym>FBal0156592==Ecol\attR<up>1</up></fbsym>
|cloning vector | recombinational | <fbsym>FBal0156591==Ecol\attR<up>2</up></fbsym>
|cloning vector | epitope tag | <fbsym>FBal0155771==T:Avic\GFP<up>YFP.Venus</up></fbsym>
CC|construct
|Gateway (Invitrogen) destination vector. <fbsym>FBmc0002891==pHVW</fbsym> and <fbsym>FBmc0002892==pHWV</fbsym> differ in
| location of epitope tag.
}

REF
{
REFM|FBrf0179058
|Murphy
|2003-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000841	CCL 1 constr	SGTP 1 composite	SYM 1 pH{101}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 319
ID|FBmc0000841
SYM|pH{101}
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0044250
|Streck et al.
|1986
ASAL|hobo\T<up>101</up>
TCH|incomplete | hobo
TCL|plasmid
TPU|characterization | hobo
DEC|in vitro isolate <fbsym>FBtp0011932==H{101}</fbsym>
}

REF
{
REFM|FBrf0044250
|Streck et al.
|1986
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000842	CCL 1 constr	SGTP 1 composite	SYM 1 pH{102}	DBA 1 -	REF 1 1	DT 1 8 Apr 1997	RESZ 319
ID|FBmc0000842
SYM|pH{102}
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0044250
|Streck et al.
|1986
ASAL|hobo\T<up>102</up>
TCH|incomplete | hobo
TCL|plasmid
TPU|characterization | hobo
DEC|in vitro isolate <fbsym>FBtp0011933==H{102}</fbsym>
}

REF
{
REFM|FBrf0044250
|Streck et al.
|1986
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000838	CCL 1 constr	SGTP 1 composite	SYM 1 pH{108}	DBA 1 -	REF 1 1	DT 1 24 Jun 1997	RESZ 566
ID|FBmc0000838
SYM|pH{108}
SYN|pH108
DT|24 Jun 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0014191==hobo\T</fbsym>

REFDSR
{
RDID|FBrf0044250
|Streck et al.
|1986
SYN|pH{108}
|pH108
ASAL|<fbsym>FBal0045893==hobo\T<up>108</up></fbsym>
TCL|plasmid
TFT|full length H-element
TPU|characterization | hobo
PRG|in vitro progenitor: EMBL4 lambda library derived from Daek strain
|in vitro progenitor: pUC9 (sequence entry L09128 | 310832 | 07 Jun 1993)
DEC|in vitro isolate <fbsym>FBtp0011934==H{108}</fbsym>
}

REF
{
REFM|FBrf0044250
|Streck et al.
|1986
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0001459	CCL 1 constr	SGTP 1 composite	SYM 1 pH{FL1+NotI}	DBA 1 -	REF 1 1	DT 1 13 May 1999	RESZ 544
ID|FBmc0001459
SYM|pH{FL1+NotI}
SYN|HFLN
DT|13 May 1999
|30 Sep 1998
CCL|constr
SGTP|composite

REFDSR
{
RDID|FBrf0069031
|Calvi
|1993
CS|NotI
SYN|HFLN
TCL|plasmid
TPU|construction intermediate
CC|construct
|Lacks selectable marker. Used in conjunction with a shuttle vector
| containing a polylinker flanked by NotI sites.
PRG|in vitro progenitor <fbsym>FBmc0000911==pH{FL1}</fbsym>
DEC|in vitro descendant <fbsym>FBmc0001460==pH{hawN}</fbsym>
|in vitro descendant <fbsym>FBms0003773==pH{harN}</fbsym>
}

REF
{
REFM|FBrf0069031
|Calvi
|1993
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000911	CCL 1 constr	SGTP 1 composite	SYM 1 pH{FL1}	DBA 1 -	REF 1 8	DT 1 8 Apr 1997	RESZ 3201
ID|FBmc0000911
SYM|pH{FL1}
TRL|6268
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 - <fbsym>FBms0000792==pBLM-mcs(K,Sc1)</fbsym>
|2 + <fbsym>FBms0002226==001173</fbsym>
|3 + <fbsym>FBms0002227==94E-0.034</fbsym>
|4 + <fbsym>FBtp0000859==H{FL1}</fbsym>
|5 + <fbsym>FBms0002228==94E-0.4</fbsym>
|6 + <fbsym>FBms0002229==001174</fbsym>
ASCO|<fbsym>FBmc0000911==pH{FL1}</fbsym>
|<fbsym>FBmc0000964==pH{haw1}</fbsym>
|<fbsym>FBmc0000963==pP{H&Dgr;2324}</fbsym>
|<fbsym>FBmc0000961==pP{HBL1}</fbsym>
|<fbsym>FBmc0000962==pP{HFS1964}</fbsym>
ASGN|<fbsym>FBgn0014191==hobo\T</fbsym>
RSQ|FBmc0000911

REFDSR
{
RDID|FBrf0049613
|Blackman et al.
|1989
ENL|SacI
ENR|SacI (inclusive)
ASAL|<fbsym>FBal0034784==hobo\T<up>+t2.96</up></fbsym>
TCH|complete | hobo
TCL|plasmid
TFT|complete rescue | <fbsym>FBal0034784==hobo\T[+t2.96]</fbsym>
|transcription unit | H\T<up>+</up>
|autonomous <fbsym>FBtp0011431==H-element</fbsym>
TPU|transposase source | <fbsym>FBgn0014191==hobo\T</fbsym> | source mobile
|transposase source | <fbsym>FBgn0014191==hobo\T</fbsym> | active in germ-line
CC|construct
|Vector assumed to be pBluescript KS(-); may be pBluescript KS(+).
PRG|in vitro progenitor: pBluescript KS(-)
|in vitro progenitor: 94E region sequences
|in vitro progenitor: <fbsym>FBtp0011431==H-element</fbsym> sequences
DEC|in vitro isolate <fbsym>FBtp0000859==H{FL1}</fbsym>
}

REFDSR
{
RDID|FBrf0049802
|Wilson et al.
|1989
TFT|bacterial selection | ampR
}

REFDSR
{
RDID|FBrf0053377
|Calvi et al.
|1991
SYN|pH{FL1}
TPU|characterization | hobo
|characterization | <fbsym>FBgn0014191==hobo\T</fbsym>
|transposase source | <fbsym>FBgn0014191==hobo\T</fbsym> | source mobile
CC|construct
|One of a series of constructs used to characterize the <fbsym>FBgn0001210==hobo</fbsym> transposable
| element and sequences required for <fbsym>FBgn0001210==hobo</fbsym> transposase activity.
|construct
|One of a series of constructs used to characterize the <fbsym>FBtp0011431==H-element</fbsym> (hobo)
| and sequences required for <fbsym>FBtp0011431==H-element</fbsym> transposase activity.
DEC|in vitro descendant <fbsym>FBmc0000961==pP{HBL1}</fbsym>
|in vitro descendant <fbsym>FBmc0000962==pP{HFS1964}</fbsym>
|in vitro descendant <fbsym>FBmc0000963==pP{H&Dgr;2324}</fbsym>
|in vitro descendant <fbsym>FBmc0000964==pH{haw1}</fbsym>
}

REFDSR
{
RDID|FBrf0065575
|Smith et al.
|1993
DEC|in vitro descendant <fbsym>FBmc0000965==pH{Lw2}</fbsym>
}

REFDSR
{
RDID|FBrf0068438
|Calvi and Gelbart
|1994
DEC|in vitro descendant <fbsym>FBmc0001376==pP{hsH\T-2}</fbsym>
|in vitro descendant <fbsym>FBmc0000968==pH{H&Dgr;2-3}</fbsym>
|in vitro descendant <fbsym>FBmc0000969==pH{H&Dgr;2-3Inv}</fbsym>
|in vitro descendant <fbsym>FBmc0000970==pH{FLN}</fbsym>
}

REFDSR
{
RDID|FBrf0069031
|Calvi
|1993
DEC|in vitro descendant <fbsym>FBmc0000910==pH{har1}</fbsym>
|in vitro descendant <fbsym>FBmc0001459==pH{FL1+NotI}</fbsym>
}

REF
{
REFM|FBrf0049613
|Blackman et al.
|1989
REFM|FBrf0049802
|Wilson et al.
|1989
REFM|FBrf0053377
|Calvi et al.
|1991
REFM|FBrf0065575
|Smith et al.
|1993
REFM|FBrf0068438
|Calvi and Gelbart
|1994
REFM|FBrf0069031
|Calvi
|1993
REFM|FBrf0090025
|Calvi
|1996.5.7
REFM|FBrf0113640
|Calvi
|1994.10.30
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000970	CCL 1 constr	SGTP 1 composite	SYM 1 pH{FLN}	DBA 1 -	REF 1 6	DT 1 24 Jun 1997	RESZ 1673
ID|FBmc0000970
SYM|pH{FLN}
TRL|~13570
DT|24 Jun 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 + <fbsym>FBms0002353==hobo5'(H5',S)</fbsym>
|2 + <fbsym>FBms0002354==001175</fbsym>
|3 + <fbsym>FBms0002355==hobo_0.85(S,H3)</fbsym>
|4 + <fbsym>FBms0003323==HindIII</fbsym>
|5 ? <fbsym>FBms0000046==ry<up>+</up>7.2(H3)</fbsym>
|6 + <fbsym>FBms0003323==HindIII</fbsym>
|7 + <fbsym>FBms0002356==hobo3'(H3,H3')</fbsym>
|8 + <fbsym>FBms0003687==pFL1-H</fbsym>
ASGN|<fbsym>FBgn0003308==ry</fbsym>
MMP|FBmc0000970
RSQ|FBmc0000970

REFDSR
{
RDID|FBrf0039004
|Spradling and Rubin
|1983
TFT|complete rescue | <fbsym>FBal0039845==ry[+t7.2]</fbsym>
|selectable marker | <fbsym>FBal0039845==ry[+t7.2]</fbsym>
}

REFDSR
{
RDID|FBrf0040064
|Rubin and Spradling
|1983
ASAL|<fbsym>FBal0039845==ry<up>+t7.2</up></fbsym>
}

REFDSR
{
RDID|FBrf0049802
|Wilson et al.
|1989
TFT|bacterial selection | ampR
}

REFDSR
{
RDID|FBrf0068438
|Calvi and Gelbart
|1994
CS|NotI
ASAL|<fbsym>FBal0045895==H\T<up>FLN</up></fbsym>
TCL|transposon | H-element | plasmid
TPU|cloning vector | transposon | hobo
PRG|in vitro progenitor <fbsym>FBmc0000117==pP{Car20}</fbsym>
|in vitro progenitor <fbsym>FBmc0000911==pH{FL1}</fbsym>
DEC|in vitro descendant <fbsym>FBmc0000968==pH{H&Dgr;2-3}</fbsym>
|in vitro descendant <fbsym>FBmc0000969==pH{H&Dgr;2-3Inv}</fbsym>
|transposon descendant <fbsym>FBtp0000913==H{FLN}</fbsym>
}

REFDSR
{
RDID|FBrf0105495
|FlyBase
|1992-
CS|NotI
}

REF
{
REFM|FBrf0039004
|Spradling and Rubin
|1983
REFM|FBrf0040064
|Rubin and Spradling
|1983
REFM|FBrf0049802
|Wilson et al.
|1989
REFM|FBrf0068438
|Calvi and Gelbart
|1994
REFM|FBrf0069031
|Calvi
|1993
REFM|FBrf0105495
|FlyBase
|1992-
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000910	CCL 1 constr	SGTP 1 composite	SYM 1 pH{har1}	DBA 1 -	REF 1 2	DT 1 24 Jun 1997	RESZ 1259
ID|FBmc0000910
SYM|pH{har1}
DT|24 Jun 1997
|21 Mar 1997
CCL|constr
SGTP|composite
ASGN|<fbsym>FBgn0003308==ry</fbsym>

REFDSR
{
RDID|FBrf0049613
|Blackman et al.
|1989
ASAL|<fbsym>FBal0039845==ry<up>+t7.2</up></fbsym>
TCL|plasmid
TFT|selectable marker | <fbsym>FBal0039845==ry[+t7.2]</fbsym>
|chimeric gene | hobo::ry::hobo
TPU|characterization | hobo\T
CC|construct
|Derived from an inversion that breaks within <fbsym>FBtp0011431==H-element</fbsym>s at 22E1-2 and
| 22F1-2 (<fbsym>FBgn0000490==dpp</fbsym>).
|report
|<fbsym>FBal0039845==ry<up>+t7.2</up></fbsym> flanked by functional <fbsym>FBtp0011431==H-element</fbsym> ends,
| allowing assay of <fbsym>H\T</fbsym> activity.
PRG|in vitro progenitor <fbsym>FBmc0000117==pP{Car20}</fbsym>
|in vitro progenitor: pUC18
|in vitro progenitor: 22E genomic sequences
|in vitro progenitor: <fbsym>FBtp0011431==H-element</fbsym> sequences
|in vitro progenitor: <fbsym>FBgn0000490==dpp</fbsym> genomic sequences
DEC|transposon descendant <fbsym>FBtp0000858==H{har1}</fbsym>
}

REFDSR
{
RDID|FBrf0069031
|Calvi
|1993
PRG|in vitro progenitor <fbsym>FBmc0000117==pP{Car20}</fbsym>
|in vitro progenitor <fbsym>FBmc0000911==pH{FL1}</fbsym>
}

REF
{
REFM|FBrf0049613
|Blackman et al.
|1989
REFM|FBrf0069031
|Calvi
|1993
}

}

# EOR

MCR
{

RETE|ID 1 FBmc0000964	CCL 1 constr	SGTP 1 composite	SYM 1 pH{haw1}	DBA 1 -	REF 1 3	DT 1 8 Apr 1997	RESZ 2019
ID|FBmc0000964
SYM|pH{haw1}
TRL|9215
DT|8 Apr 1997
|21 Mar 1997
CCL|constr
SGTP|composite
SEG|1 - <fbsym>FBms0000792==pBLM-mcs(K,Sc1)</fbsym>
|2 + <fbsym>FBms0002226==001173</fbsym>
|3 + <fbsym>FBms0002227==94E-0.034</fbsym>
|4 + <fbsym>FBms0002443==hobo5'(H5',R1)</fbsym>
|5 + <fbsym>FBms0000726==001105</fbsym>
|6 + <fbsym>FBms0000515==w<up>+mC</up>4.1(Sp)</fbsym>
|7 - <fbsym>FBms0000726==001105</fbsym>
|8 + <fbsym>FBms0002344==hobo3'(R1,H3')</fbsym>
|9 + <fbsym>FBms0002229==001174</fbsym>
ASCO|<fbsym>FBmc0000911==pH{FL1}</fbsym>
|<fbsym>FBmc0000964==pH{haw1}</fbsym>
|<fbsym>FBmc0000963==pP{H&Dgr;2324}</fbsym>
|<fbsym>FBmc0000961==pP{HBL1}</fbsym>
|<fbsym>FBmc0000962==pP{HFS1964}</fbsym>
ASGN|<fbsym>FBgn0003996==w</fbsym>
RSQ|FBmc0000964

REFDSR
{
RDID|FBrf0048982
|Pirrotta
|1988
ASAL|<fbsym>FBal0028610==w<up>+mC</up></fbsym>
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
|partial rescue | mini-gene::<fbsym>FBal0028610==w[+mC]</fbsym>
}

REFDSR
{
RDID|FBrf0049802
|Wilson et al.
|1989
TFT|bacterial selection | ampR
}

REFDSR
{
RDID|FBrf0053377
|Calvi et al.
|1991
ENL|SacI
ENR|SacI (inclusive)
SYN|pH{haw1}
TCL|plasmid
TFT|selectable marker | <fbsym>FBal0028610==w[+mC]</fbsym>
TPU|characterization | hobo\T
CC|construct
|One of a series of constructs used to characterize the <fbsym>FBgn0001210==hobo</fbsym> transposable
| element and sequences required for <fbsym>FBgn0001210==hobo</fbsym> transposase activity.
|construct
|Additional